SOS CHROMOTEST, A DIRECT ASSAY OF INDUCTION OF AN SOS FUNCTION IN ESCHERICHIA-COLI K-12 TO MEASURE GENOTOXICITY
SOS CHROMOTEST, A DIRECT ASSAY OF INDUCTION OF AN SOS FUNCTION IN ESCHERICHIA-COLI K-12 TO MEASURE GENOTOXICITY
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DOI：
10.1073/pnas.79.19.5971
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发表时间：
1982-01-01
期刊：
影响因子：
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通讯作者：
HOFNUNG, M
中科院分区：
文献类型：
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作者：
QUILLARDET, P;HUISMAN, O;HOFNUNG, M
SOS显色试验是一种用于检测DNA损伤剂的细菌试验。这是一种基于这些试剂诱导SOS功能sfiA的比色测定法，其表达水平通过sfiA：：lacZ操纵子融合来监测。反应迅速（几小时），不需要试验菌株存活。各种化学品的剂量反应曲线包括线性区域。将该区域的斜率作为SOS诱导效力的量度。将SOS显色试验中测定的SOS诱导效价的许多已知遗传毒性物质与沙门氏菌试验（mutatest）中测定的致突变效价进行比较，发现超过7个数量级的显著定量相关性。SOS显色试验的灵敏度（检测到的最低量）与突变试验的灵敏度相同，通常比噬菌体诱导试验（inductest）的灵敏度高4-40倍。从实践的角度来看，这些观察结果有助于验证SOS显色试验作为检测遗传毒素，特别是遗传毒性致癌物的试验。在突变试验中测量的致突变效力反映了SOS功能的诱导水平，并且大多数遗传毒素是细菌中SOS反应的诱导剂。[This研究可能适用于人类健康。
The SOS chromotest, a bacterial test for detecting DNA-damaging agents, is evaluated. It is a colorimetric assay based on the induction by these agents of the SOS function sfiA, whose level of expression is monitored by means of a sfiA::lacZ operon fusion. The response is rapid (a few hours), and does not require survival of the tester strain. Dose-response curves for various chemicals include a linear region. The slope of this region is taken as a measure of the SOS inducing potency. Comparison for a number of substances of known genotoxicity of the SOS inducing potency determined in the SOS chromotest with the mutagenic potency determined in the Salmonella assay (mutatest) revealed a striking quantitative correlation over more than 7 orders of magnitude. The sensitivity of the SOS chromotest (lowest amount detected) is equal to that of the mutatest and generally 4-40 times higher than that of a phage induction assay (inductest). From a practical point of view these observations contribute to the validation of the SOS chromotest as a test for detecting genotoxins and in particular genotoxic carcinogens. Mutagenic potency measured in the mutatest reflects the level of induction of an SOS function and most genotoxins are inducers of the SOS response in bacteria. [This study may be applicable to human health.].