C-PR neuron of Aplysia has differential effects on "feeding" cerebral interneurons, including myomodulin-positive CBI-12
C-PR neuron of Aplysia has differential effects on "feeding" cerebral interneurons, including myomodulin-positive CBI-12
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DOI：
10.1152/jn.1999.81.2.521
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发表时间：
1999-02-01
影响因子：
2.5
通讯作者：
Kupfermann, I
中科院分区：
文献类型：
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作者：
Hurwitz, I;Perrins, R;Kupfermann, I
海兔完成进食动作之前的头部抬起和食欲中心动机状态的其他方面是通过 C-PR 神经元的兴奋来促进的。食物刺激会激活 C-PR 以及一小部分脑颊中间神经元 (CBI)。我们希望确定 C-PR 的激发是否对各种 CBI 产生不同的影响，或者可能统一影响所有 CBI，就像参与产生广泛的未分化唤醒状态的神经元所预期的那样。我们发现，当 C-PR 被触发时，它会对各种 CBI 产生各种各样的影响。 C-PR 的激发引起了对新识别的 CBI (CBI-12) 的兴奋性输入，其体细胞位于先前识别的 CBI-2 附近的 M 簇中。 CBI-12 与 CBI-2 具有某些相同的特性，包括相似的形态和驱动颊神经节节律活动的能力。与 CBI-2 不同，CBI-12 表现出肌调节蛋白免疫反应性。此外，当 C-PR 被激发时，CBI-12 接收到多突触电压依赖性慢速激励，而 CBI-2 接收相对较少的输入。 C-PR 还多突触兴奋其他 CBI，包括 CBI-1 和 CBI-8/9，但对 CBI-S 产生抑制。此外，C-PR 的激发抑制了 CBI-5/6 的平台电位。数据表明，C-PR 的活动可能会促进大脑-颊侧中间神经元的一个子集的活动，这些中间神经元可能与抬头姿势期间发生的摄取行为有关。 C-PR 还抑制一些大脑颊间神经元，这些中间神经元可能参与不需要 C-PR 活性的行为，甚至可能干扰其他摄食行为，例如低头时发生的拒绝或放牧行为。
Head lifting and other aspects of the appetitive central motive state that precedes consummatory feeding movements in Aplysia is promoted by excitation of the C-PR neuron. Food stimuli activate C-PR as well as a small population of cerebral-buccal interneurons (CBIs). We wished to determine if firing of C-PR produced differential effects on the various CBIs or perhaps affected all the CBIs uniformly as might be expected for a neuron involved in producing a broad undifferentiated arousal state. We found that when C-PR was fired, it produced a wide variety of effects on various CBIs. Firing of C-PR evoked excitatory input to a newly identified CBI (CBI-12) the soma of which is located in the M cluster near the previously identified CBI-2. CBI-12 shares certain properties with CBI-2, including a similar morphology and a capacity to drive rhythmic activity of the buccal-ganglion. Unlike CBI-2, CBI-12 exhibits myomodulin immunoreactivity. Furthermore when C-PR is fired, CBI-12 receives a polysynaptic voltage-dependent slow excitation, whereas, CBI-2 receives relatively little input. C-PR also polysynaptically excites other CBIs including CBI-1 and CBI-8/9 but produces inhibition in CBI-S. In addition, firing of C-PR inhibits plateau potentials in CBI-5/6. The data suggest that activity of C-PR may promote the activity of one subset of cerebral-buccal interneurons, perhaps those involved in ingestive behaviors that occur during the head-up posture. C-PR also inhibits some cerebral-buccal interneurons that may be involved in behaviors in which C-PR activity is not required or may even interfere with other feeding behaviors such as rejection or grazing, that occur with the head down.