Synthesis and oligonucleotide incorporation of fluorescent cytosine analogue tC: a promising nucleic acid probe
Synthesis and oligonucleotide incorporation of fluorescent cytosine analogue tC: a promising nucleic acid probe
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荧光胞嘧啶类似物 tC 的合成和寡核苷酸掺入：一种有前途的核酸探针
DOI：
10.1038/nprot.2007.80
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发表时间：
2007
期刊：
影响因子：
14.8
通讯作者：
L. Wilhelmsson
中科院分区：
文献类型：
--
作者：
P. Sandin;P. Lincoln;T. Brown;L. Wilhelmsson
三环胞嘧啶 tC 是一种荧光碱基类似物，具有优异的特性，可用于研究核酸的内在特征以及核酸与其他分子之间的相互作用。其独特的荧光特性以及掺入后对核酸整体结构和动力学的微不足道的影响使得 tC 在荧光共振能量转移和各向异性测量中特别有趣。我们在这里描述了用于 DNA 固相合成的 tC 标准单体形式 tC 亚磷酰胺的直接合成，以及随后将其掺入寡核苷酸中。 tC 亚磷酰胺的全合成大约需要 8 天，其掺入和随后的寡核苷酸纯化又需要一天。
The tricyclic cytosine, tC, is a fluorescent base analogue with excellent properties for investigating intrinsic characteristics of nucleic acid as well as interactions between nucleic acids and other molecules. Its unique fluorescence properties and insignificant influence on overall structure and dynamics of nucleic acid after incorporation makes tC particularly interesting in fluorescence resonance energy transfer and anisotropy measurements. We here describe a straightforward synthesis of the standard monomer form of tC for DNA solid-phase synthesis, the tC phosphoramidite, and its subsequent incorporation into oligonucleotides. The total synthesis of the tC phosphoramidite takes approximately 8 days and its incorporation and the subsequent oligonucleotide purification an additional day.
影响因子：
2.9
作者：
Stivers, JT;Pankiewicz, KW;Watanabe, KA
通讯作者：
Watanabe, KA
影响因子：
14.9
作者：
Stivers, JT
通讯作者：
Stivers, JT