Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions
Tunable pores for measuring concentrations of synthetic and biological nanoparticle dispersions
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DOI：
10.1016/j.bios.2011.09.040
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发表时间：
2012-01-15
影响因子：
12.6
通讯作者：
Vogel, Robert
中科院分区：
文献类型：
--
作者：
Roberts, G. Seth;Yu, Sam;Vogel, Robert
扫描离子遮挡传感 (SIOS) 是一种使用可调孔来检测单个纳米级物体通过的技术，用于快速、准确和直接测量合成和生物纳米颗粒浓度。与流式细胞术或库尔特计数器等其他直接计数技术相比，SIOS 能够表征更小的颗粒，并且直接计数避免了浊度测量所需的近似值。在直径为 210-710 nm 的聚苯乙烯颗粒模型系统中进行的测量表明，事件频率与施加的压力和浓度呈线性关系，并且测量的浓度与颗粒类型和尺寸无关。演示了外部校准和免校准测量方法。然后应用SIOS测量直径约1μm的杆状病毒包涵体和直径约600nm的海洋光合蓝藻原绿球藻的浓度。测定的浓度分别与显微镜计数（平均浓度之间有 17% 的差异）和流式细胞术（平均浓度之间有 6% 的差异）计数的结果非常吻合。 (C) 2011 Elsevier B.V. 保留所有权利。
Scanning ion occlusion sensing (SIOS), a technique that uses a tunable pore to detect the passage of individual nano-scale objects, is applied here for the rapid, accurate and direct measurement of synthetic and biological nanoparticle concentrations. SIOS is able to characterize smaller particles than other direct count techniques such as flow cytometry or Coulter counters, and the direct count avoids approximations such as those necessary for turbidity measurements. Measurements in a model system of 210-710 nm diameter polystyrene particles demonstrate that the event frequency scales linearly with applied pressure and concentration, and that measured concentrations are independent of particle type and size. Both an external-calibration and a calibration-free measurement method are demonstrated. SIOS is then applied to measure concentrations of Baculovirus occlusion bodies, with a diameter of similar to 1 mu m, and the marine photosynthetic cyanobacterium Prochlorococcus, with a diameter of similar to 600 nm. The determined concentrations agree well with results from counting with microscopy (a 17% difference between the mean concentrations) and flow cytometry (6% difference between the mean concentrations), respectively. (C) 2011 Elsevier B.V. All rights reserved.