Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels.
Genomic insights into strategies used by Xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels.
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DOI：
10.1186/1471-2164-13-658
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发表时间：
2012-11-21
期刊：
影响因子：
4.4
通讯作者：
Cociancich S
中科院分区：
文献类型：
--
作者：
Pieretti I;Royer M;Barbe V;Carrere S;Koebnik R;Couloux A;Darrasse A;Gouzy J;Jacques MA;Lauber E;Manceau C;Mangenot S;Poussier S;Segurens B;Szurek B;Verdier V;Arlat M;Gabriel DW;Rott P;Cociancich S
白纹黄单胞菌引起甘蔗的一种致死性病害--叶枯病。X.与黄单胞菌属的其他物种相比，白念珠菌具有独特的致病机制、生态学和分类学。例如，该物种产生一种称为albicidin的有效DNA促旋酶抑制剂，其主要负责诱导疾病症状;其栖息地仅限于木质部;并且该物种表现出很大的变异性。高致病性X染色体组全序列的第一份手稿。albilineans菌株GPE PC 73专门关注与另一种木质部限制性黄单胞菌科（Xanthomonadaceae）-苛养木杆菌（Xylella fastidiosa）共有的独特基因组特征。目前的手稿上相同的基因组序列的目的是描述所有其他致病性相关的基因组特征的X。albilineans，并比较，使用抑制性消减杂交（SSH），基因组特征的两个菌株的致病性不同。比较基因组分析表明，大多数已知的致病因子从其他黄单胞菌属物种是保守的X。黄单胞菌属的其他植物病原性物种的"火炮"的两个主要决定因素的显著缺乏，即黄原胶生物合成基因簇和III型分泌系统Hrp（过敏反应和致病性）。X的基因组特征。还揭示了可能有助于该病原菌对甘蔗木质部导管的特异性适应的albilineans。SSH实验鉴定了三种高致病性菌株共有的20个基因，但在致病性较低的菌株中缺失。这20个基因包括4个ABC转运蛋白基因、1个甲基接受趋化蛋白基因和1个氧化还原酶基因，可能在致病性中起关键作用。除了我们的比较基因组分析和SSH实验所揭示的假设蛋白质外，没有任何基因可能参与X的任何攻击或防御机制。albilineans的鉴定，假设X.与其他致病性黄单胞菌属物种相比，白念珠菌具有减少的火炮。因此，特别关注X的基因组特征。使其更有能力躲避甘蔗监视系统或抵抗甘蔗防御系统。本研究证实X. albilineans是黄单胞菌属中一个非常独特的物种，为更好地了解这种破坏性甘蔗病原菌的致病性开辟了新的视角。
Xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. X. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of Xanthomonas. For example, this species produces a potent DNA gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. A first manuscript on the complete genome sequence of the highly pathogenic X. albilineans strain GPE PC73 focused exclusively on distinctive genomic features shared with Xylella fastidiosa—another xylem-limited Xanthomonadaceae. The present manuscript on the same genome sequence aims to describe all other pathogenicity-related genomic features of X. albilineans, and to compare, using suppression subtractive hybridization (SSH), genomic features of two strains differing in pathogenicity. Comparative genomic analyses showed that most of the known pathogenicity factors from other Xanthomonas species are conserved in X. albilineans, with the notable absence of two major determinants of the “artillery” of other plant pathogenic species of Xanthomonas: the xanthan gum biosynthesis gene cluster, and the type III secretion system Hrp (hypersensitive response and pathogenicity). Genomic features specific to X. albilineans that may contribute to specific adaptation of this pathogen to sugarcane xylem vessels were also revealed. SSH experiments led to the identification of 20 genes common to three highly pathogenic strains but missing in a less pathogenic strain. These 20 genes, which include four ABC transporter genes, a methyl-accepting chemotaxis protein gene and an oxidoreductase gene, could play a key role in pathogenicity. With the exception of hypothetical proteins revealed by our comparative genomic analyses and SSH experiments, no genes potentially involved in any offensive or counter-defensive mechanism specific to X. albilineans were identified, supposing that X. albilineans has a reduced artillery compared to other pathogenic Xanthomonas species. Particular attention has therefore been given to genomic features specific to X. albilineans making it more capable of evading sugarcane surveillance systems or resisting sugarcane defense systems. This study confirms that X. albilineans is a highly distinctive species within the genus Xanthomonas, and opens new perpectives towards a greater understanding of the pathogenicity of this destructive sugarcane pathogen.
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DOI：
10.1099/00221287-136-1-51
发表时间：
1990-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
BIRCH, RG;PEMBERTON, JM;BASNAYAKE, WV
通讯作者：
BASNAYAKE, WV
影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
影响因子：
6.7
作者：
Blanch, Maria;Legaz, Maria-Estrella;Vicente, C.
通讯作者：
Vicente, C.
影响因子：
3.2
作者：
Champoiseau, P.;Daugrois, J. -H.;Rott, P.
通讯作者：
Rott, P.
影响因子：
4.5
作者：
Champoiseau, P.;Rott, P.;Daugrois, J. -H.
通讯作者：
Daugrois, J. -H.