Murine photoplethysmography for in vivo estimation of vascular gold nanoshell concentration.
Murine photoplethysmography for in vivo estimation of vascular gold nanoshell concentration.
复制标题
鼠光电体积描记法用于体内估计血管金纳米壳浓度。
DOI：
10.1117/1.3454374
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发表时间：
2010
影响因子：
3.5
通讯作者：
D. O'Neal
中科院分区：
文献类型：
--
作者：
G. Michalak;Glenn P. Goodrich;J. Schwartz;William D James;D. O'Neal
临床上迫切需要监测用于癌症治疗的循环纳米颗粒的静脉内递送和生物利用度。这项工作提出了使用光电体积描记法的非侵入性实时估计血管金纳米壳浓度在小鼠受试者。我们开发了一种脉冲光度计，能够准确地测量小鼠的光体积描记图，并确定在805和940 nm之间的光学消光的脉动变化的比率，通常被称为R。选择这些波长以对应于金纳米壳的消光性质。在轻度麻醉下进行六次30秒测量（5分钟、2、4、6、8、10小时），以观察当纳米颗粒从循环中清除时R的变化。我们的模型描述了R和通过离体分光光度计和仪器中子活化分析测量的纳米颗粒浓度之间的线性拟合（R（2）= 0.85）。这证明了该技术在支持临床纳米颗粒疗法中的实用性。
There is an urgent clinical need to monitor the intravenous delivery and bioavailability of circulating nanoparticles used in cancer therapy. This work presents the use of photoplethysmography for the noninvasive real-time estimation of vascular gold nanoshell concentration in a murine subject. We develop a pulse photometer capable of accurately measuring the photoplethysmogram in mice and determining the ratio of pulsatile changes in optical extinction between 805 and 940 nm, commonly referred to as R. These wavelengths are selected to correspond to the extinction properties of gold nanoshells. Six 30-s measurements (5 min, 2, 4, 6, 8, 10 h) are taken under light anesthesia to observe the change in R as the nanoparticles clear from the circulation. Our model describes the linear fit (R(2)=0.85) between R and the concentration of nanoparticles measured via ex vivo spectrophotometric and instrumental neutron activation analysis. This demonstrates the utility of this technique in support of clinical nanoparticle therapies.