The mechanism of action of the two‐layer (Euro‐Collins' solution/perfluorochemical) cold storage method in canine pancreas preservation
The mechanism of action of the two‐layer (Euro‐Collins' solution/perfluorochemical) cold storage method in canine pancreas preservation
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DOI：
10.1111/tri.1992.5.s1.391
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发表时间：
1992-01
影响因子：
3.1
通讯作者：
Y. Kuroda;Y. Fujino;A. Morita;Y. Tanioka;Y. Suzuki;T. Kawamura;Y. Ku;Y. Saitoh
中科院分区：
文献类型：
--
作者：
Y. Kuroda;Y. Fujino;A. Morita;Y. Tanioka;Y. Suzuki;T. Kawamura;Y. Ku;Y. Saitoh
为了阐明双层[欧洲柯林斯溶液（EC）/全氟化合物（PFC）]冷藏方法在保存胰腺中的作用机制，在EC（组1）、双层EC/PFC方法（组2）和双层EC/PFC方法（组2）中简单冷藏保存24和48小时后，在自体胰腺移植犬模型中检查胰腺活力和三磷酸腺苷（ATP）的组织浓度。双层，EC + 2, 4 二硝基苯酚 (DNP)/PFC，方法（第 3 组）。 DNP 是氧化磷酸化的解偶联剂。移植后维持正常血糖至少5天被认为是成功的保存。保存24 h后，第1、2、3组功能成功率分别为100%（4/4）、100%（5/5）和80%（4/5）。第三组中的五只狗中有一只死于与胰腺无关的原因。第 2 组的 ATP 组织浓度显着高于第 1 组（7.47 ± 0.47 gmol/g 干重 vs 1.41 ± 0.53 gmol/g 干重，P < 0.01），第 3 组的 ATP 组织浓度显着低于第 2 组（1.25 ± 0.37 gmol/g 干重 vs 7.47 ± 0.47 gmol/g 干重，P < 0.01）。 0.01）。显然，ATP 并不是 EC 中犬胰腺成功保存 24 小时的重要因素，因为无论 ATP 组织浓度如何，除了第 3 组中的 5 个移植物之一外，所有胰腺移植物在保存 24 小时后仍然存活。另一方面，保存48小时后，第1组、第2组和第3组的功能成功率分别为0%（0/4）、100%（4/4）和0%（0/3）。第 2 组的 ATP 组织浓度显着高于第 1 组（7.91 ± 1.21 gmol/g 干重 vs 1.21 ± 0.314 tmol/g 干重，P < 0.01），第 3 组的 ATP 组织浓度显着低于第 2 组（0.61 ± 0.07 gmol/g 干重 vs 7.91 ± 1.21 µmol/g 干重，P < 0.01）。 0.01）。很明显，通过在 EC 中简单冷藏（第 1 组）无法成功保存胰腺 48 小时，而双层方法（第 2 组）通过增加 ATP 组织浓度使胰腺保存 48 小时成为可能。然而，DNP（组 3）抑制 ATP 的合成以及双层方法对胰腺 48 小时保存的有效性。很明显，在保存过程中维持高 ATP 组织浓度对于通过双层法在 EC 中成功保存犬胰腺超过 48 小时至关重要。我们得出的结论是，在双层法保存过程中，胰腺有足够的氧气供应，可以产生足够的 ATP 来维持细胞完整性，并可以改善胰腺的保存。
To clarify the mechanism of action of a two‐layer [Euro‐Collins' solution (EC)/perfluorochemical (PFC) ] cold storage method in the preservation of the pancreas, pancreatic viability and tissue concentrations of adenosine triphosphate (ATP) were examined in the canine model of pancreatic autotransplantation after preservation for 24 and 48 h by simple cold storage in EC (group 1), the two‐layer, EC/PFC, method (group 2) and the two‐layer, EC + 2, 4 dinitrophenol (DNP)/PFC, method (group 3). DNP is an uncoupler of oxidative phosphorylation. Maintenance of normoglycemia for at least 5 days after transplantation was considered a successful preservation. After preservation for 24 h, the functional success rates of groups 1, 2 and 3 were 100% (4/4), 100% (5/5) and 80% (4/5) respectively. One of five dogs in group 3 died of a cause unrelated to the pancreas. ATP tissue concentrations in group 2 were significantly higher than in group 1 (7.47 ± 0.47 gmol/g dry weight vs 1.41 ± 0.53 gmol/g dry weight, P < 0.01) and ATP tissue concentrations in group 3 were significantly lower than in group 2 (1.25 ± 0.37 gmol/g dry weight vs 7.47 ± 0.47 gmol/g dry weight, P < 0.01). It was apparent that ATP was not an essential factor for successful 24‐hour preservation of the canine pancreas in EC because all the pancreatic grafts except one of five grafts in group 3 remained viable after preservation for 24 h, regardless of ATP tissue concentrations. On the other hand, after preservation for 48 h, the functional success rates for groups 1, 2 and 3 were 0% (0/4), 100% (4/4) and 0% (0/3) respectively. ATP tissue concentrations in group 2 were significantly higher than in group 1 (7.91 ± 1.21 gmol/g dry weight vs 1.21 ± 0.314tmol/g dry weight, P < 0.01) and ATP tissue concentrations in group 3 were significantly lower than in group 2 (0.61 ± 0.07 gmol/g dry weight vs 7.91 ± 1.21 µmol/g dry weight, P < 0.01). It was clear that preservation of the pancreas for 48 h was unsuccessful by simple cold storage in EC (group 1) and the two‐layer method (group 2) made preservation for 48 h possible by increasing ATP tissue concentrations. However, DNP (group 3) inhibited the synthesis of ATP and the effectiveness of the two‐layer method for 48‐hour preservation of the pancreas. It was clear that maintenance of high ATP tissue concentrations during preservation was essential for the successful preservation of the canine pancreas in EC by the two‐layer method for more than 48 h. We concluded that an adequate supply of oxygen to the pancreas during preservation by the two‐layer method led to sufficient production of ATP to maintain cellular integrity and permitted the improvement of pancreatic preservation.