The antagonistic effect between STAT1 and Survivin and its clinical significance in gastric cancer
The antagonistic effect between STAT1 and Survivin and its clinical significance in gastric cancer
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STAT1与Survivin在胃癌中的拮抗作用及其临床意义
DOI：
10.3892/ol.2011.423
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发表时间：
2012-01-01
期刊：
影响因子：
2.9
通讯作者：
Liu, Lijiang
中科院分区：
文献类型：
--
作者：
Deng, Hao;Zhen, Hongyan;Liu, Lijiang
在既往研究中，我们观察到STAT1和Survivin与胃癌组织呈负相关，并且IFN-γ-STAT1通路和Survivin在胃癌中的功能与报道的其他类型癌症相同。本研究利用SGC7901胃癌细胞系和83例胃癌标本来证实STAT1与Survivin之间的关系，以及这种关系在胃癌中的临床意义。使用 IFN-γ、STAT1 和 Survivin 反义寡核苷酸 (ASON) 敲低 SGC7901 细胞中的表达。采用免疫细胞化学和图像分析方法检测STAT1和Survivin蛋白表达。制备胃癌组织微阵列并采用免疫组化方法进行检测。数据通过Spearman等级相关分析、χ2检验和Cox多元回归分析进行分析。在 SGC7901 细胞系中，ASON 敲低 IFN-γ、STAT1 和 Survivin 的表达后，观察到 STAT1 和 Survivin 之间存在拮抗作用。在胃癌组织中，STAT1与浸润深度呈负相关（p<0.05），而在Survivin蛋白表达阴性的胃癌组织中，STAT1与浸润深度呈负相关（p<0.05）。此外，在 STAT1 蛋白表达阴性的组织中，Survivin 与 N 期呈负相关 (p<0.05)。采用病理和分子标志物进行Cox多元回归分析，发现浸润深度和N分期是预后因素（p<0.05）。另一方面，在Survivin蛋白表达阴性的组织中，Cox的多元回归分析显示分化类型和STAT1蛋白表达是预后因素(p<0.05)。 STAT1与Survivin在胃癌中存在拮抗作用，这种拮抗作用在胃癌中具有临床意义。
In previous studies, we observed that STAT1 and Survivin correlated negatively with gastric cancer tissues, and that the functions of the IFN-γ-STAT1 pathway and Survivin in gastric cancer are the same as those reported for other types of cancer. In this study, the SGC7901 gastric cancer cell line and 83 gastric cancer specimens were used to confirm the relationship between STAT1 and Survivin, as well as the clinical significance of this relationship in gastric cancer. IFN-γ and STAT1 and Survivin antisense oligonucleotides (ASONs) were used to knock down the expression in SGC7901 cells. The protein expression of STAT1 and Survivin was tested by immunocytochemical and image analysis methods. A gastric cancer tissue microarray was prepared and tested by immunohistochemical methods. Data were analyzed by the Spearman's rank correlation analysis, the χ2 test and Cox's multivariate regression analysis. Upon knockdown of IFN-γ, STAT1 and Survivin expression by ASON in the SGC7901 cell line, an antagonistic effect was observed between STAT1 and Survivin. In gastric cancer tissues, STAT1 showed a negative correlation with depth of invasion (p< 0.05) in gastric cancer tissues exhibiting a negative Survivin protein expression. Furthermore, in tissues exhibiting a negative STAT1 protein expression, Survivin correlated negatively with N stage (p< 0.05). Pathological and molecular markers were used to conduct Cox's multivariate regression analysis, and depth of invasion and N stage were found to be prognostic factors (p< 0.05). On the other hand, in tissues exhibiting a negative Survivin protein expression, Cox's multivariate regression analysis revealed that the differentiation type and STAT1 protein expression were prognostic factors (p< 0.05). There is an antagonistic effect between STAT1 and Survivin in gastric cancer, and this antagonistic effect is of clinical significance in gastric cancer.