In vitro photochemical cataract in mice lacking copper-zinc superoxide dismutase
In vitro photochemical cataract in mice lacking copper-zinc superoxide dismutase
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DOI：
10.1016/s0891-5849(01)00651-7
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发表时间：
2001-09-15
影响因子：
7.4
通讯作者：
Marklund, SL
中科院分区：
文献类型：
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作者：
Behndig, A;Karlsson, K;Marklund, SL
我们在这里使用可见光照射和核黄素作为光敏剂，在体外模型中评估缺乏胞质铜锌超氧化物歧化酶（CuZn-SOD）的小鼠白内障的形成。将野生型和 CuZn-SOD-null 小鼠的分离培养晶状体与 10 μM 核黄素预孵育 24 小时后，用日光荧光灯照射 1.5 小时。每天通过数字图像分析和眼部分期评估白内障的形成，并在 5 天后测定 Rb-86 的摄取和晶状体的水含量。使用光泽精衍生的化学发光法测定野生型和 CuZn-SOD-null 小鼠新鲜分离的晶状体中的基础超氧化物浓度，并使用直接分光光度法测定鼠晶状体中所有三种 SOD 同工酶的酶活性。细胞质 CuZn-SOD 占小鼠晶状体总 SOD 活性的 90%。 CuZu-SOD-null 晶状体显示出双倍的基础超氧化物浓度，并且在本模型中更容易发生光化学白内障，与野生型小鼠的晶状体相比，具有更多的混浊度、更多的水合作用和更少的 Rb-86 摄取。我们得出的结论是，CuZn-SOD 是晶状体中重要的超氧化物清除剂，并且可能具有防止白内障形成的保护作用。 (C) 2001 爱思唯尔科学公司。
We here evaluate cataract formation in mice lacking the cytosolic copper-zinc superoxide dismutase (CuZn-SOD) in an in vitro model using irradiation with visible light and riboflavin as a photosensitizing agent. Isolated, cultured lenses from wild-type and CuZn-SOD-null mice were irradiated for 1.5 h by a daylight fluorescent light after preincubation with 10 muM riboflavin for 24 h. Cataract formation was evaluated daily with digital image analysis and ocular staging, and after 5 d Rb-86 uptake and water contents of the lenses were determined. Basal superoxide concentrations in freshly isolated lenses from wild-type and CuZn-SOD-null mice were determined with lucigenin-derived chemiluminescense, and enzymatic activities of all three SOD isoenzymes in the murine lens were determined with a direct spectrophotometric method. The cytosolic CuZn-SOD accounts for 90% of the total SOD activity of the murine lens. CuZu-SOD-null lenses showed a doubled basal superoxide concentration, and were more prone to develop photochemical cataract in the present model with more opacification, more hydration, and less Rb-86 uptake than lenses from wild-type mice. We conclude that CuZn-SOD is an important superoxide scavenger in the lens, and that it may have a protective role against cataract formation. (C) 2001 Elsevier Science Inc.