Identification and mutation analysis of DOC-1R, a DOC-1 growth suppressor-related gene.
Identification and mutation analysis of DOC-1R, a DOC-1 growth suppressor-related gene.
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DOI：
10.1006/bbrc.1999.0148
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发表时间：
1999-02
影响因子：
3.1
通讯作者：
X. Zhang;H. Tsao;T. Tsuji;S. Minoshima;J. Mcbride;P. Majewski;R. Todd;N. Shimizu;D. Wong;D. Housman;F. Haluska
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文献类型：
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作者：
X. Zhang;H. Tsao;T. Tsuji;S. Minoshima;J. Mcbride;P. Majewski;R. Todd;N. Shimizu;D. Wong;D. Housman;F. Haluska
肿瘤抑制基因MEN 1和包括CCND 1/细胞周期蛋白D1/PRAD 1在内的几个癌基因定位于染色体11 q13。然而，分子和细胞遗传学分析表明，在这一染色体区域存在第二个肿瘤抑制基因座。我们已经确定了一个新的基因，从染色体11 q13，它编码的126个氨基酸的蛋白质共享一个整体的57%的同一性与p12（DOC-1）的蛋白质编码的DOC-1基因，人类同源物的仓鼠假定的肿瘤抑制DOC-1（在口腔癌-1删除）。因此，我们将该新基因命名为DOC-1相关的DOC-1 R。染色体荧光原位杂交证实了细胞遗传学定位。北方印迹分析表明，该基因在所有检测的组织中均有表达。DOC-1 R蛋白在细胞内的定位不均一。RT-PCR-SSCP分析未能检测到有害突变的DOC-1 R转录在4个癌前口腔角质形成细胞系和20个不同的肿瘤类型，经常港口洛杂合性缺失在染色体11 q13的癌细胞系。
The tumor suppressor gene MEN1 and several oncogenes including CCND1/cyclin D1/PRAD1 map to chromosome 11q13. However, molecular and cytogenetic analysis suggests the presence of a second tumor suppressor locus at this chromosome region. We have identified a novel gene from chromosome 11q13, which encodes a protein of 126 amino acids sharing an overall 57% identity with the p12(DOC-1) protein encoded by the DOC-1 gene, the human homolog of hamster putative tumor suppressor doc-1 (deleted in oral cancer-1). We therefore designated the novel gene as DOC-1R for DOC-1-related. The cytogenetic location was confirmed by chromosome fluorescent in situ hybridization. Northern blot analysis indicated that it was expressed in all the tissues examined. DOC-1R protein showed heterogeneous subcellular localization. RT-PCR-SSCP analysis failed to detect deleterious mutations of the DOC-1R transcript in four premalignant oral keratinocyte lines and 20 different cancer cell lines from tumor types which frequently harbor LOH at chromosome 11q13.