Virus-like vesicles and extracellular DNA produced by hyperthermophilic archaea of the order Thermococcales
Virus-like vesicles and extracellular DNA produced by hyperthermophilic archaea of the order Thermococcales
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DOI：
10.1016/j.resmic.2008.04.015
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发表时间：
2008-06-01
影响因子：
2.6
通讯作者：
Forterre, Patrick
中科院分区：
文献类型：
--
作者：
Soler, Nicolas;Marguet, Evelyne;Forterre, Patrick
对嗜热古生菌(热球菌目)的培养物进行了电子显微镜和荧光染色分析，以确定病毒样颗粒的存在。我们发现，大多数热球菌和热球菌能产生不同类型的球状膜泡和不寻常的丝状结构。细胞DNA可以强烈地与囊泡结合，并在荧光显微镜下显示为荧光点，这表明一些在生态学研究中被认为是病毒的颗粒可能是与细胞外DNA相关的囊泡。囊泡制剂中的DNA对DNA酶处理和热变性具有显著的抗性，这表明与囊泡的结合可能是决定DNA在自然环境中稳定性的重要因素。(C)2008年爱思唯尔·马森公司。版权所有。
Cultures of hyperthermophilic archaea (order Thermococcales) have been analyzed by electron microscopy and epifluorescence staining for the presence of virus-like particles. We found that most strains of Thermococcus and Pyrococcus produce various types of spherical membrane vesicles and unusual filamentous structures. Cellular DNA can be strongly associated with vesicles and appears as fluorescent dots by epifluorescence microscopy, suggesting that some particles assumed to be viruses in ecological studies might instead be vesicles associated with extracellular DNA. DNA in vesicle preparations is remarkably resistant to DNase treatment and thermodenaturation, indicating that association with vesicles could be an important factor determining DNA stability in natural environments. (C) 2008 Elsevier Masson SAS. All rights reserved.