Biophysical and morphological changes in the stratum corneum lipids induced by UVB irradiation
Biophysical and morphological changes in the stratum corneum lipids induced by UVB irradiation
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DOI：
10.1016/j.jdermsci.2006.05.012
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发表时间：
2006-10-01
影响因子：
4.6
通讯作者：
Zhou, Xiao Jun
中科院分区：
文献类型：
--
作者：
Jiang, Shao Jun;Chen, Jie Yu;Zhou, Xiao Jun
背景：紫外线照射引起表皮的多种反应，包括晒伤细胞形成、表皮增生和表皮通透性屏障破坏。目的：探讨中波紫外线照射对小鼠角质层细胞间脂质的影响。方法：采用单次UVB剂量（0.15 J/cm(2)）照射成年无毛小鼠，采用傅里叶变换红外光谱（FT-IR）研究其对角质层脂质生物物理变化的影响，经皮失水（TEWL）测量屏障功能，四氧化二钌后固定电镜观察角质层形态学变化。结果：FT-IR光谱研究表明，单次UVB照射后第3-4天，对称和不对称拉伸峰分别在2850和2920 cm（-1）附近向更高波数偏移，反映了脂质烃链运动自由度的增加，称为脂质无序化。此外，单次UVB照射也导致TEWL显著增加，TEWL在2天后开始增加，在第4天达到峰值。电镜观察发现细胞间区形态异常明显，包括角质层与颗粒层交界面层状颗粒及其含量异常，角质层内细胞核持续存在。而且，脂质片片分离的碎片性，层叠中脂质片片数量过多，两者都存在。角质层宽的间隙内可见延长、增大的陷窝和细胞外旋体。结论：角质层脂质在生物物理和形态学上的变化可能反映了UVB照射对表皮通透性屏障的扰动机制。(c) 2006年日本皮肤病调查学会。爱思唯尔爱尔兰有限公司出版。版权所有。
Background: UV irradiation induces a variety of responses in the epidermis, including sunburn cell formation, epidermal hyperplasia, and epidermal permeability barrier disruption.Objective: The aim of present study was to assess the effects of UVB irradiation in the intercellular lipids in murine stratum corneum.Methods: Adult hairless mice were exposed to a single UVB dose (0.15 J/cm(2)), the Fourier transform infrared (FT-IR) spectroscopic study was performed to investigate the effect on the biophysical changes in the stratum corneum lipids, barrier function was monitored by transepidermal water loss (TEWL) measurement, and the morphological alterations of stratum corneum was examined by electron microscopy using ruthenium tetroxide postfixation.Results: The FT-IR spectroscopic study revealed that there was the shift to higher wavenumbers of the symmetric and asymmetric stretching peaks near 2850 and 2920 cm(-1) respectively at days 3-4 after a single UVB irradiation, reflecting to the increase in motional freedom of lipids hydrocarbon chains, call as disordering of lipids. Moreover, A single UVB irradiation also caused a significant increase in TEWL, the increase in TEWL began after 2 days and peaked at day 4. Electron microscopic observations revealed that marked morphological abnormalities in the intercellular domains, including abnormal profile of lamellar granules and its contents at the interface between stratum corneum and stratum granulosum and the persistence of the nuclei in the stratum corneum. Moreover, the separated fragmentary lipid lamellae, excessive numbers of lamellae in stacks, both the. elongated and enlarged Lacuna as well as the extracellular whorls were present within the widen space of the stratum corneum.Conclusion: The both of biophysical and morphological changes of the stratum corneum lipids may reflect to the mechanisms of perturbation of the epidermal permeability barrier induced by UVB irradiation. (c) 2006 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved.