Preliminary Study on Linkage Mapping Based on Microsatellite DNA and AFLP Markers Using Homozygous Clonal Fish in Ayu (Plecoglossus altivelis)
Preliminary Study on Linkage Mapping Based on Microsatellite DNA and AFLP Markers Using Homozygous Clonal Fish in Ayu (Plecoglossus altivelis)
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DOI：
10.1007/s10126-003-0026-4
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发表时间：
2004-06
影响因子：
3
通讯作者：
Tomohisa Watanabe;Hironobu Fujita;Kimio Yamasaki;Shingo Seki;N. Taniguchi
中科院分区：
文献类型：
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作者：
Tomohisa Watanabe;Hironobu Fujita;Kimio Yamasaki;Shingo Seki;N. Taniguchi
用正常二倍体雄性与纯合克隆雌性杂交，获得香鱼的定位参考家系（F1）。利用191个AFLP标记和4个微卫星DNA标记构建了一张遗传连锁图谱。在36个连锁群中共定位了178个位点，总长度为1659.6cM，约占总基因组的77.3%~ 81.8%。由于这些标记在基因组上随机分布，因此它们在构建宽范围连锁图方面表现出很高的效率。
A mapping referential family (F1) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.