Simultaneous spatial and temporal focusing of femtosecond pulses
Simultaneous spatial and temporal focusing of femtosecond pulses
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DOI：
10.1364/opex.13.002153
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发表时间：
2005-03-21
期刊：
影响因子：
3.8
通讯作者：
Xu, C
中科院分区：
文献类型：
--
作者：
Zhu, GH;van Howe, J;Xu, C
为了提高多光子成像中的信噪比，我们研究并演示了飞秒脉冲的时空同步聚焦技术。这一概念是通过将脉冲的光谱分量空间分离成“彩虹光束”并仅在物镜透镜的空间焦点处重新组合这些分量来实现的。因此，时间脉冲宽度成为距离的函数，其中最短脉冲宽度限于空间焦点。我们开发了解析表达式来描述这种方法，并通过实验证明了其可行性。飞秒脉冲的同时空间和时间聚焦的概念具有极大的潜力，以显着减少在多光子显微镜，从根本上限制了在高散射生物标本的成像深度的背景激发。(C)2005年，美国光学学会。
We study and demonstrate the technique of simultaneous spatial and temporal focusing of femtosecond pulses, with the aim to improve the signal-to-background ratio in multiphoton imaging. This concept is realized by spatially separating spectral components of pulses into a "rainbow beam" and recombining these components only at the spatial focus of the objective lens. Thus, temporal pulse width becomes a function of distance, with the shortest pulse width confined to the spatial focus. We developed analytical expressions to describe this method and experimentally demonstrated the feasibility. The concept of simultaneous spatial and temporal focusing of femtosecond pulses has the great potential to significantly reduce the background excitation in multiphoton microscopy, which fundamentally limits the imaging depth in highly scattering biological specimens. (C) 2005 Optical Society of America.