Point of use SERS-based lateral flow test for pathogenic infections
Point of use SERS-based lateral flow test for pathogenic infections
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基于 SERS 的病原感染侧向层析检测的使用点
DOI：
10.1117/12.2675805
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Hassanain W
中科院分区：
文献类型：
--
作者：
Hassanain W
横向流动免疫分析是基于纸质的检测，可用于在使用点检测不同的致病生物标记物。传统上，当样品中存在生物标记物时，标记有金纳米颗粒的检测抗体在测试线/点上的目标抗原和捕获抗体之间形成夹心免疫分析。它们根据试条上的金纳米颗粒的外观提供快速、直观和是/否的答案。然而，侧向流动试验的灵敏度较低，当生物标志物浓度低于试验的视觉阈值时，特别是在感染的早期阶段，可能会导致假阴性结果。他们也不能准确地量化生物标记物的浓度，这对监测感染的进展或治疗的有效性很重要。因此，侧向流动测试应与读出系统相结合，该系统便于使用，并能够准确、灵敏和快速地量化测试结果。在这项工作中，我们讨论了在一个平台上将表面增强拉曼散射技术与横向流动测试相结合，以提高测试的灵敏度和量化能力，同时保持横向流动测试提供的用户友好和使用点特性。我们已经将这种方法应用于艰难梭状芽胞杆菌和SARS-COV-2的检测，作为病原性感染的示范者，使用测试中的新识别元件作为概念验证。
Lateral flow immunoassays are paper-based tests that can be used to detect different pathogenic biomarkers at the point-of-use. Conventionally, detection antibodies labelled with gold nanoparticles form sandwich immunoassays between the target antigen and capture antibody on the test line/spot when the biomarker is present in a sample. They provide rapid, visual and yes/no answers based on the appearance of the gold nanoparticles on the strip. However, lateral flow tests suffer from poor sensitivity which can lead to false negative results when the biomarker concentration is below the visual threshold of the test, especially at early infection stages. They also can’t accurately quantify the biomarker concentration which is important in monitoring the progression of an infection or the effectiveness of a treatment. Accordingly, lateral flow test should be combined with a read-out system that is convenient for points of use and could enable the accurate, sensitive and rapid quantification of the test results. In this work, we discuss the integration of surface enhanced Raman scattering technique with the lateral flow test in one platform to improve the test sensitivity and quantification capability, while maintaining the user-friendly and point-of-use features the lateral flow test provides. We have applied this approach for the detection ofClostridioides difficileand SARS-COV-2, as demonstrators for pathogenic infections, using new recognition elements in the test as a proof-of-concept.
影响因子：
4.2
作者：
Hassanain, Waleed A.;Spoors, Julia;Graham, Duncan
通讯作者：
Graham, Duncan