Taurine Attenuates Streptococcus uberis-Induced Bovine Mammary Epithelial Cells Inflammation via Phosphoinositides/Ca2+ Signaling
Taurine Attenuates Streptococcus uberis-Induced Bovine Mammary Epithelial Cells Inflammation via Phosphoinositides/Ca2+ Signaling
复制标题
牛磺酸通过磷酸肌醇/Ca2信号传导减轻乳房链球菌诱导的牛乳腺上皮细胞炎症
DOI：
10.3389/fimmu.2019.01825
复制
发表时间：
2019-08-07
影响因子：
7.3
通讯作者：
Miao, Jinfeng
中科院分区：
文献类型：
--
作者：
Li, Ming;Xi, Panpan;Miao, Jinfeng
牛磺酸可能通过调节细胞内Ca2+水平来减轻由uberis链球菌感染引起的炎症损伤。然而，潜在的机制仍不清楚。感染导致与Ca2+信号密切相关的磷酸肌苷（pi）的颠覆。为了研究牛磺酸是否通过pi /Ca2+系统调节炎症，使用了牛磺酸（β -丙氨酸）siTauT、siPAT1、siPLC、siCaN、siPKC的竞争性抑制剂以及PLC （U73122）、PKC （RO31-8220）和CaN （FK 506）的抑制剂。结果表明，牛磺酸将细胞间先天免疫的细胞外营养信号传递给磷酸肌苷，而不需要进入细胞质，同时在炎症期间调节细胞内Ca2+水平。uberis感染的Ca2+-PKC α - nf -kappa B和Ca2+-CaM-CaN-NFAT信号通路以及牛磺酸的调节作用都遵循pi /Ca2+系统的激活。这些数据增加了我们对多功能营养素牛磺酸减轻uberis感染引起的炎症反应机制的认识，并为该疾病的预防提供了理论支持。
Taurine may alleviate the inflammatory injury induced by Streptococcus uberis (S. uberis) infection by regulating intracellular Ca2+ levels. However, the underlying mechanisms remain unclear. Infection leads to subversion of phosphoinositides (PIs) which are closely related to Ca2+ signaling. In order to investigate whether taurine regulates inflammation by means of PIs/Ca2+ systems, competitive inhibitors of taurine (beta-alanine) siTauT, siPAT1, siPLC, siCaN, siPKC, and inhibitors of PLC (U73122), PKC (RO31-8220), and CaN (FK 506) were used. The results indicate that taurine transfers the extracellular nutrient signal for intercellular innate immunity to phosphoinositides without a need to enter the cytoplasm while regulating intracellular Ca2+ levels during inflammation. Both the Ca2+-PKC alpha-NF-kappa B, and Ca2+-CaM-CaN-NFAT signaling pathways of S. uberis infection and the regulatory roles of taurine follow activation of PIs/Ca2+ systems. These data increase our understanding on the mechanisms of multifunctional nutrient, taurine attenuated inflammatory responses caused by S. uberis infection, and provide theoretical support for the prevention of this disease.