TOPOGRAPHY OF TRANSCRIPTION FACTOR COMPLEXES ON THE SACCHAROMYCES-CEREVISIAE 5-S RNA GENE
TOPOGRAPHY OF TRANSCRIPTION FACTOR COMPLEXES ON THE SACCHAROMYCES-CEREVISIAE 5-S RNA GENE
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DOI：
10.1016/0022-2836(92)90315-b
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发表时间：
1992-12-20
影响因子：
5.6
通讯作者：
GEIDUSCHEK, EP
中科院分区：
文献类型：
--
作者：
BRAUN, BR;BARTHOLOMEW, B;GEIDUSCHEK, EP
酵母 RNA 聚合酶 III 转录因子 (TFIII) A、C 和 B 在 5 S rRNA 基因上的组成蛋白位置已通过位点特异性 DNA 蛋白光交联确定。与之前分析的 tRNA 基因进行比较表明，尽管内部启动子元件的位置不同，但在这两个基因上组装了相似的核蛋白结构。这种同源性的主要特征是 TFIIIC 95 kDA 亚基的位置，它与 tRNA 基因的 boxA 启动子元件相关。在 5S rRNA 基因上，在没有 aboxAsequence 的情况下，95 kDa 亚基占据相同的空间，尽管下游存在 30 个碱基对的 abox A 样序列。之前未被认为是 TFIIIC 组成部分的 90 kDa 成分被特异性定位于 5 S rRNA 基因的 3' 端。
Locations of component proteins of yeast RNA polymerase III transcription factors (TFIII) A, C and B on a 5 S rRNA gene have been determined by site-specific DNA-protein photocrosslinking. Comparison with a previously analyzed tRNA gene shows that similar nucleoprotein structures assemble on these two genes despite their differently located internal promoter elements. A principal signature of this homology is the placement of the 95 kDA subunit of TFIIIC, which associates with theboxApromoter element of the tRNA gene. On the 5 S rRNA gene, the 95 kDa subunit occupies the same space in the absence of aboxAsequence, and despite the presence of abox A-like sequence 30 base-pairs further downstream. A 90 kDa component that was not previously recognized as an integral part of TFIIIC has been specifically located at the 3′ end of the 5 S rRNA gene.