Nanoscale Curvature Promotes High Yield Spontaneous Formation of Cell-Mimetic Giant Vesicles on Nanocellulose Paper
Nanoscale Curvature Promotes High Yield Spontaneous Formation of Cell-Mimetic Giant Vesicles on Nanocellulose Paper
复制标题
纳米级曲率促进纳米纤维素纸上仿细胞巨型囊泡的高产率自发形成
DOI：
10.1021/acsami.0c14485
复制
发表时间：
2020
影响因子：
9.5
通讯作者：
Subramaniam, Anand Bala
中科院分区：
文献类型：
--
作者：
Pazzi, Joseph;Subramaniam, Anand Bala
迄今为止，用于将磷脂膜组装成细胞样巨单层囊泡（GUV）的技术使用平面表面，并且需要施加电场或溶解的分子以获得足够的产率。在这里，我们提出了使用纳米纤维素纸，这是由缠绕的圆柱形纳米纤维组成的表面，以促进GUV的简易和高产率的组装。与现有的表面辅助组装技术相比，使用纳米纤维素纸导致成本降低高达100 000倍，同时提高产量。使用大数据集共聚焦显微镜定量测量的产量和尺寸分布阐明了组装的机制。我们提出了一个热力学的“萌芽和合并”，BNM，模型提供了一个统一的解释，从不同的几何形状和化学表面的GUV的产量和大小的差异。BNM模型通过平衡过渡到表面附着球形芽的表面附着膜的一部分的弹性、粘附力和边缘能量来考虑由于出芽引起的自由能的变化。该模型表明，GUV的形成是自发的亲水性表面上的缠结的圆柱形纳米纤维与纳米纤维素纤维的尺寸相似。这项工作的进展了解的表面性质的GUV组装的影响。它还解决了目前阻碍GUV作为药物递送、合成细胞制造和人工组织大规模组装载体的实际障碍。
To date, techniques for the assembly of phospholipid films into cell-like giant unilamellar vesicles (GUVs) use planar surfaces and require the application of electric fields or dissolved molecules to obtain adequate yields. Here, we present the use of nanocellulose paper, which are surfaces composed of entangled cylindrical nanofibers, to promote the facile and high yield assembly of GUVs. Use of nanocellulose paper results in up to a 100 000-fold reduction in costs while increasing yields compared to extant surface-assisted assembly techniques. Quantitative measurements of yields and the distributions of sizes using large data set confocal microscopy illuminates the mechanism of assembly. We present a thermodynamic “budding and merging”, BNM, model that offers a unified explanation for the differences in the yields and sizes of GUVs obtained from surfaces of varying geometry and chemistry. The BNM model considers the change in free energy due to budding by balancing the elastic, adhesion, and edge energies of a section of a surface-attached membrane that transitions into a surface-attached spherical bud. The model reveals that the formation of GUVs is spontaneous on hydrophilic surfaces consisting of entangled cylindrical nanofibers with dimensions similar to nanocellulose fibers. This work advances understanding of the effects of surface properties on the assembly of GUVs. It also addresses practical barriers that currently impede the promising use of GUVs as vehicles for the delivery of drugs, for the manufacturing of synthetic cells, and for the assembly of artificial tissues at scale.