The islets of Langerhans. Preface.
The islets of Langerhans. Preface.
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朗格汉斯岛。
DOI：
10.1016/j.ultsonch.2008.10.007
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发表时间：
2010
影响因子：
--
通讯作者：
Md. Shadidul Islam
中科院分区：
文献类型：
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作者：
Md. Shadidul Islam
我们以前开发了人工启动子，激活响应X射线照射。用1.0MHz的超声波处理引起细胞内氧化应激，发现激活这些启动子中的一些，尽管程度较低。这些启动子中最敏感的一个表现出强度和持续时间依赖性激活超声。此外，当存在N-乙酰半胱氨酸时，其通过超声处理的活化被减弱，表明细胞内氧化应激参与活化机制。还发现对X射线照射敏感的改进的启动子对超声处理更敏感。其中1例经1.0MHz、1.0W/cm 2超声处理60 s后增强最明显，增强6.0倍。这种增强也减弱与N-乙酰半胱氨酸的存在。当将具有最敏感启动子的稳定转染的HeLa细胞移植到小鼠上并进行超声处理时，启动子的荧光素酶活性平均增加至1.35倍，尽管与对照相比没有统计学显著性。虽然基因调控在体内超声处理还不清楚，这是第一次报告人工构建的启动子响应超声。
We previously developed artificial promoters that were activated in response to X-ray irradiation. Sonication with 1.0MHz ultrasound that causes intracellular oxidative stress was found to activate some of these promoters though to lesser degrees. The most sensitive one among these promoters showed intensity- and duration-dependent activations by sonication. In addition, its activation by sonication was attenuated when N-acetyl cysteine was present, suggesting the involvement of intracellular oxidative stress in the activation mechanism. Improved promoters for sensitivity to X-ray irradiation were also found more sensitive to sonication. The most improved one showed 6.0 fold enhancement after sonication with 1.0MHz ultrasound at 1.0W/cm2for 60s. This enhancement was also attenuated with the presence of N-acetyl cysteine. When stably transfected HeLa cells with the most sensitive promoter were transplanted on to mice and sonicated, luciferase activity by the promoter increased to 1.35 fold in average though it was not statistically significant compared to control. Although gene regulation in vivo by sonication was not clear, this is the first report on artificially constructed promoters responsive to ultrasound.