Insulin-Like Growth Factor-I Improves Chondrogenesis of Predifferentiated Human Umbilical Cord Mesenchymal Stromal Cells
Insulin-Like Growth Factor-I Improves Chondrogenesis of Predifferentiated Human Umbilical Cord Mesenchymal Stromal Cells
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DOI：
10.1002/jor.20848
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发表时间：
2009-08-01
影响因子：
2.8
通讯作者：
Detamore, Michael S.
中科院分区：
文献类型：
--
作者：
Wang, Limin;Detamore, Michael S.
人脐带间充质干细胞（Human umbilical cord mesenchymal stromal cells，hUCMSCs）是一种非常有吸引力的组织工程细胞来源，与其他成体干细胞来源相比具有许多优点，如在体外具有很强的扩增能力。体外和广泛的可用性。这项为期6周的研究旨在检验以下假设：在3周时间点从软骨形成转化生长因子-β 3（TGF-β 3）转换为合成代谢胰岛素样生长因子-I（IGF-I）将比单独使用TGF-β 3产生更多的软骨样基质。将hUCMSC接种到聚乙醇酸（PGA）支架中，然后在含有TGF-β 3的软骨形成培养基中培养3周。然后将TGF-β 3处理的hUCMSC暴露于四种不同条件之一再3周：（1）继续在软骨形成培养基中，（2）对照培养基（无TGF-β 3），（3）具有10 ng/ml IGF-I的对照培养基，或（4）具有100 ng/ml IGF-I的对照培养基。与继续使用TGF-β 3相比，转换为IGF-I增加了胶原蛋白的产生，并且进一步增加了胶原蛋白11型基因表达和免疫染色。总之，在第3周从TGF-β 3到IGF-I的转变导致软骨样细胞外基质的显著增加，证实了我们的假设。(C)2009骨科研究学会。出版社：Wiley Periodicals，Inc. J Orthop Res 27：1109-1115，2009
Human umbilical cord mesenchymal stromal cells (hUCMSCs) are an attractive cell Source for tissue engineering with numerous advantages over other adult stem cell sources, such as great expansion ability in. vitro and extensive availability. The objective of this 6-week study was to test the hypothesis that switching from chondrogenic transforming growth factor-beta3 (TGF-beta 3) to anabolic insulin-like growth factor-I (IGF-I) at the 3-week time point would produce more cartilage-like matrix than TGF-beta 3 alone. hUCMSCs were seeded into polyglycolic acid (PGA) scaffolds and then cultured in chondrogenic medium containing TGF-beta 3 for 3 weeks. The TGF-beta 3-treated hUCMSCs were then exposed for 3 more weeks to one of four different conditions: (1) continued in chondrogenic medium, (2) control medium (no TGF-beta 3), (3) control medium with 10 ng/ml IGF-I, or (4) control medium with 100 ng/ml IGF-I. Compared to continuing With TGF-beta 3, switching to IGF-I increased collagen production, and furthermore increased both collagen type 11 gene expression and immunostaining. In conclusion, the shift from TGF-beta 3 to IGF-I at week 3 resulted in a significant increase of cartilage-like extracellular-matrix, confirming our hypothesis. (C) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1109-1115, 2009