T cells subsets and cytokines in allergic and non-allergic children. II. Analysis and IL-5 and IL-10 mRNA expression and protein production.
T cells subsets and cytokines in allergic and non-allergic children. II. Analysis and IL-5 and IL-10 mRNA expression and protein production.
复制标题
过敏和非过敏儿童的 T 细胞亚群和细胞因子。
作者：
H. Koning;H. Koning;H. J. Neijens;H. J. Neijens;M. Baert;M. Baert;A. P. Oranje;A. P. Oranje;H. Savelkoul;H. Savelkoul
白介素5(IL-5)对IL-4诱导的免疫球蛋白E(IgE)合成有促进作用。此外，IL-5在嗜酸性粒细胞的分化、募集、激活和存活中起重要作用。IL-10对干扰素-γ的产生有下调作用，具有较强的抗炎活性。因此，我们分析了过敏性和非过敏性哮喘儿童、特应性皮炎儿童和健康对照组儿童T细胞在IL-5和IL-10mRNA表达和蛋白产生方面是否存在差异。与健康对照组相比，过敏性和非过敏性哮喘儿童和特应性皮炎儿童不同T细胞部分的IL-5mRNA表达和蛋白产生显著增加。这表明IL-5不仅参与了过敏反应，而且在非过敏性哮喘的炎症过程中也发挥了作用。有趣的是，过敏性和非过敏性哮喘和特应性皮炎患儿纯化的T细胞IL-10mRNA的表达与健康对照组相比显著降低。在外周血单个核细胞(PBMC)部分，IL-10mRNA的表达在四组之间相似。我们推测，这种T细胞来源的IL-10表达减少导致这些疾病的炎症过程缺乏免疫抑制。然而，不能排除单核细胞来源的IL-10的作用。
Interleukin 5 (IL-5) has an enhancing effect on IL-4 induced immunoglobulin E (IgE) synthesis. Furthermore, IL-5 plays an important role in the differentiation, recruitment, activation and survival of eosinophils. IL-10 has a downmodulating effect on interferon gamma (IFN-gamma) production and can exert strong anti-inflammatory activities. Therefore, we analysed whether differences were present in IL-5 and IL-10 mRNA expression and protein production between T cells of children with allergic and non-allergic asthma, atopic dermatitis and healthy control children. We demonstrated significant increases in IL-5 mRNA expression and protein production in different T cell fractions of children with allergic and non-allergic asthma and children with atopic dermatitis as compared to healthy controls. This indicates that IL-5 is not only involved in allergy, but also plays a role in the inflammatory process of non-allergic asthma. Interestingly, IL-10 mRNA expression by purified T cells of children with allergic and non-allergic asthma and children with atopic dermatitis was strongly decreased as compared with that of healthy controls. In the peripheral blood mononuclear cell (PBMC) fraction, IL-10 mRNA expression was comparable between the four groups. We hypothesize that this decreased T cell derived IL-10 expression results in a lack of immunosuppression of the inflammatory process in these diseases. However, a role of monocyte derived IL-10 cannot be ruled out.