Elucidation of the relationship of BNIP3 expression to gemcitabine chemosensitivity and prognosis
Elucidation of the relationship of BNIP3 expression to gemcitabine chemosensitivity and prognosis
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DOI：
10.3748/wjg.v13.i34.4593
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发表时间：
2007-09-14
影响因子：
4.3
通讯作者：
Horii, Akira
中科院分区：
文献类型：
--
作者：
Ishida, Masaharu;Sunamura, Makoto;Horii, Akira
目的：为了探讨BNIP 3在胰腺癌发病机制中的意义，我们分析了BNIP 3的表达与胰腺癌患者生存率的关系，以及胰腺癌细胞株对化疗药物的敏感性，特别是对一线抗肿瘤药物吉西他滨的敏感性。为了比较BNIP 3的表达水平与对吉西他滨的耐药性，对8个胰腺癌细胞系进行吉西他滨处理，并使用定量实时RT-PCR方法来评估BNIP 3的表达。免疫组化分析也进行了22例胰腺癌标本的BNIP 3表达和生存率之间的关系进行了研究。结果：虽然没有显着的正相关性BNIP 3 mRNA水平和吉西他滨化疗敏感性观察，胰腺癌细胞株是敏感的吉西他滨治疗往往表现出高水平的BNIP 3表达。相反，BNIP 3表达的缺失与吉西他滨耐药无关。我们进一步比较了原发性胰腺癌切除标本中BNIP 3的表达谱与患者预后的关系，发现预后良好和BNIP 3低表达的趋势。结论：高水平的BNIP 3表达不能用作吉西他滨化疗敏感性的预测因素之一，并且一些尚不为人知的因素将不得不填补精确预测胰腺癌对吉西他滨的化学敏感性的差距。然而，BNIP 3表达可能对胰腺癌患者的预后预测有影响。(c)2007年WJG。All rights reserved.
AIM: To evaluate the significance of BNIP3 in the pathogenesis of pancreatic cancer, we analyzed the relationship between the expression of BNIP3 and survival rate of the patients with pancreatic cancer, or chemosensitivities in pancreatic cancer cell lines, particularly for gemcitabine, the first-line anti-tumor drug for pancreatic cancer.METHODS: To compare the expression level of BNIP3 with the resistance to gemcitabine, eight pancreatic cancer cell lines were subjected to gemcitabine treatment and the quantitative real-time RT-PCR method was used to evaluate BNIP3 expression. Immunohistochemical analysis was also performed using 22 pancreatic cancer specimens to study relationship between BNIP3 expression and survival rate.RESULTS: Although no significantly positive association between BNIP3 mRNA level and gemcitabine chemosensitivity was observed, pancreatic canter cell lines that were sensitive to gemcitabine treatment tended to show high levels of BNIP3 expression. The converse, an absence of BNIP3 expression, was not correlated with gemcitabine resistance. We further compared the BNIP3 expression profiles of resected primary pancreatic cancer specimens with the prognosis of the patients, and found a tendency of favorable prognosis and low BNIP3 expression.CONCLUSION: High levels of BNIP3 expression cannot be used as one of the predicting factors for gemcitabine chemosensitivity, and some yet to be known factors will have to fill the gap for the accurate prediction of pancreatic cancer chemosensitivity to gemcitabine. However, BNIP3 expression may have an impact on prediction of prognosis of patients with pancreatic cancer. (c) 2007 WJG. All rights reserved.