MiR-375 Has Contrasting Effects on Newcastle Disease Virus Growth Depending on the Target Gene
MiR-375 Has Contrasting Effects on Newcastle Disease Virus Growth Depending on the Target Gene
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MiR-375 对新城疫病毒生长具有不同的影响，具体取决于目标基因
DOI：
10.7150/ijbs.25106
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发表时间：
2019-01-01
影响因子：
9.2
通讯作者：
Yang, Zengqi
中科院分区：
文献类型：
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作者：
Wang, Xinglong;Jia, Yanqing;Yang, Zengqi
microRNA通过翻译抑制或mRNA降解来调节转录后基因表达。它们在病毒感染和宿主抗感染过程中都发挥着重要作用。我们使用小RNA测序方法发现miR-375在纽卡斯尔病病毒（NDV）感染的鸡胚胎内脏组织中显著上调。进一步的研究表明，miR-375的过表达通过靶向NDV的M基因而显著降低了DF-1细胞中强毒NDV F48 E9和弱毒NDV La Sota的复制。有趣的是，miR-375有另一个靶点ELAVL 4，它调节鸡纤维细胞的细胞周期进程并降低NDV的增殖。此外，miR-375可以通过其在培养基中的分泌来影响旁观者细胞。我们的结果表明，miR-375是NDV的抑制剂，但也可以通过降低其靶ELAVL 4的表达来增强NDV的生长。这些结果强调了microRNA在病毒感染调控中的复杂作用。
MicroRNAs regulate post-transcriptional gene expression via either translational repression or mRNA degradation. They have important roles in both viral infection and host anti-infection processes. We discovered that the miR-375 is significantly upregulated in Newcastle disease virus (NDV)-infected chicken embryonic visceral tissues using a small RNA sequencing approach. Further research revealed that the overexpression of miR-375 markedly decreases the replication of the velogenic NDV F48E9 and the lentogenic NDV La Sota by targeting the M gene of NDV in DF-1 cells. Interestingly, miR-375 has another target, ELAVL4, which regulates chicken fibrocyte cell cycle progression and decreases NDV proliferation. In addition, miR-375 can influence bystander cells by its secretion in culture medium. Our results indicated that miR-375 is an inhibitor of NDV, but can also enhance NDV growth by reducing the expression of its target ELAVL4. These results emphasize the complex roles of microRNAs in the regulation of viral infections.