Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome
Map-based isolation of the leaf rust disease resistance gene Lr10 from the hexaploid wheat (Triticum aestivum L.) genome
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DOI：
10.1073/pnas.2435133100
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发表时间：
2003-12-09
影响因子：
11.1
通讯作者：
Keller, B
中科院分区：
文献类型：
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作者：
Feuillet, C;Travella, S;Keller, B
小麦基因库中已鉴定出50多个抗叶锈病基因，其中大量基因已被广泛应用于育种。在50个Lr基因中，除了最近克隆的Lr 21之外，所有基因仅从其表型和/或图谱位置已知。多年来，分子工作的问题，在大（1.6 × 10（10）bp），高度重复（80%），和六倍体面包小麦（Triticum aestivum L.）基因组阻碍了图位克隆。在这里，我们报告的分离Lr基因Lr 10从六倍体小麦的亚基因组图位克隆和单倍型研究相结合的属小麦。Lr 10是位于1AS染色体上的单拷贝基因。它编码具有N-末端结构域的CC-NBS-LRR型蛋白，该蛋白处于多样化选择下。当在转基因小麦植物中过表达时，Lr 10赋予增强的对叶锈病的抗性。Lr 10与拟南芥中的RPM 1以及水稻和大麦中的抗性基因类似物具有相似性，但与基于Southern分析的其他小麦Lr基因没有密切关系。我们的结论是，基于图位克隆的基因的农艺学上的重要性，在六倍体小麦现在是可行的，开辟前景的分子面包小麦改良通过转基因策略和诊断等位基因检测。
More than 50 leaf rust resistance W) genes against the fungal pathogen Puccinia triticina have been identified in the wheat gene pool, and a large number of them have been extensively used in breeding. Of the 50 Lr genes, all are known only from their phenotype and/or map position except for Lr21, which was cloned recently. For many years, the problems of molecular work in the large (1.6 x 10(10) bp), highly repetitive (80%), and hexaploid bread wheat (Triticum aestivum L.) genome have hampered map-based cloning. Here, we report the isolation of the Lr gene Lr10 from hexaploid wheat by using a combination of subgenome map-based cloning and haplotype studies in the genus Triticum. Lr10 is a single-copy gene on chromosome 1AS. It encodes a CC-NBS-LRR type of protein with an N-terminal domain, which is under diversifying selection. When overexpressed in transgenic wheat plants, Lr10 confers enhanced resistance to leaf rust. Lr10 has similarities to RPM1 in Arabidopsis thaliana and to resistance gene analogs in rice and barley, but is not closely related to other wheat Lr genes based on Southern analysis. We conclude that map-based cloning of genes of agronomic importance in hexaploid wheat is now feasible, opening perspectives for molecular bread wheat improvement trough transgenic strategies and diagnostic allele detection.