Pomegranate Intake Protects Against Genomic Instability Induced by Medical X-rays In Vivo in Mice.
Pomegranate Intake Protects Against Genomic Instability Induced by Medical X-rays In Vivo in Mice.
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DOI：
10.1080/01635581.2016.1225104
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发表时间：
2016-11
期刊：
影响因子：
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通讯作者：
Reliene R
中科院分区：
文献类型：
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作者：
Nallanthighal S;Shirode AB;Judd JA;Reliene R
电离辐射（IR）是一种公认的人类致癌物质。在医疗程序中越来越多地使用红外线使年辐射剂量增加了一倍，并可能增加癌症风险。基因组不稳定性是IR诱导的癌症中的中间病变。我们研究了石榴提取物（PE）是否抑制X射线诱导的基因组不稳定性。小鼠口服PE并暴露于2戈伊的X射线剂量。PE摄入抑制X射线诱导的外周血DNA双链断裂（DSB）和骨髓染色体损伤。我们假设PE介导的对X射线诱导的损伤的保护可能是由于DSB修复和抗氧化酶的上调和/或谷胱甘肽（GSH）水平的增加。我们发现，在PE处理的小鼠的肝脏中，DSB修复基因的表达没有改变（Nbs 1和Rad 50）或减少（Mre 11、DNA-PKcs、Ku 80、Rad 51、Rad 52和Brca 2）。同样，抗氧化酶的mRNA水平降低（Gpx 1，Cat和Sod 2）或不改变（HO-1和Sod 1）作为PE处理的函数。相比之下，PE治疗的小鼠与IR曝光和不显示较高的肝脏GSH浓度比对照组。因此，石榴多酚的摄入与抑制X射线诱导的基因组不稳定性和GSH升高有关，这可能会降低癌症风险。
Ionizing radiation (IR) is a well-documented human carcinogen. The increased use of IR in medical procedures has doubled the annual radiation dose and may increase cancer risk. Genomic instability is an intermediate lesion in IR-induced cancer. We examined whether pomegranate extract (PE) suppresses genomic instability induced by x-rays. Mice were treated orally with PE and exposed to an x-ray dose of 2 Gy. PE intake suppressed x-ray-induced DNA double strand breaks (DSBs) in peripheral blood and chromosomal damage in bone marrow. We hypothesized that PE-mediated protection against x-ray-induced damage may be due to upregulation of DSB repair and antioxidant enzymes and/or increase in glutathione (GSH) levels. We found that expression of DSB repair genes was not altered (Nbs1 and Rad50) or was reduced (Mre11, DNA-PKcs, Ku80, Rad51, Rad52 and Brca2) in the liver of PE treated mice. Likewise, mRNA levels of antioxidant enzymes were reduced (Gpx1, Cat and Sod2) or were not altered (HO-1 and Sod1) as a function of PE treatment. In contrast, PE treated mice with and without IR exposure displayed higher hepatic GSH concentrations than controls. Thus, ingestion of pomegranate polyphenols is associated with inhibition of x-ray-induced genomic instability and elevated GSH, which may reduce cancer risk.