Sequence analysis of a glycoprotein D gene homolog within the unique short segment of the EHV-1 genome.
Sequence analysis of a glycoprotein D gene homolog within the unique short segment of the EHV-1 genome.
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EHV-1 基因组独特短片段内糖蛋白 D 基因同源物的序列分析。
DOI：
10.1016/0042-6822(91)90021-3
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发表时间：
1991
期刊：
影响因子：
3.7
通讯作者：
O'Callaghan,DJ
中科院分区：
文献类型：
--
作者：
Flowers,CC;Eastman,EM;O'Callaghan,DJ
对马疱疹病毒1型（EHV-1）基因组三分之一的独特短（Us）片段的DNA序列分析显示，其开放阅读框（ORF）的翻译序列与单纯疱疹病毒（HSV）1型和2型的糖蛋白D以及伪狂犬病毒（PRV）糖蛋白50（gD等价物）具有显著的同源性。EHV-1 gD同源物的ORF位于pSZ-4 BamHI/lKpnl片段内（图谱单位为0.865至0.872和0.869至0.884），能够编码385个氨基酸（43，206分子量）的多肽。核苷酸序列的分析揭示了一个完整的转录单位，包括CAAT和TATA元件和多聚腺苷酸化的信号。预测的蛋白质表现出典型的跨膜蛋白的功能：一个疏水的N-末端信号序列，其次是一个可能的切割位点，四个潜在的Winked糖基化位点，和一个疏水的跨膜结构域附近的羧基末端，其次是一个带电的膜锚序列。
DNA sequence analysis of one-third of the unique short (Us) segment of the equine herpesvirus type 1 (EHV-1) genome revealed an open reading frame (ORF) whose translated sequence exhibits significant homology to glycoprotein D of herpes simplex virus (HSV) types 1 and 2 and to pseudorabies virus (PRV) glycoprotein 50, the gD equivalent. The ORF of the EHV-1 gD homolog lies within the pSZ-4BamHI/lKpnl fragment (map units 0.865 to 0.872 and 0.869 to 0.884) and is capable of encoding a polypeptide of 385 amino acids (43,206 molecular weight). Analysis of the nucleotide sequence revealed a complete transcriptional unit including CAAT and TATA elements and signals for polyadenylation. The predicted protein exhibits features typical of a transmembrane protein: a hydrophobic N-terminal signal sequence followed by a probable cleavage site, four potential Winked glycosylation sites, and a hydrophobic membrane-spanning domain near the carboxyl terminus followed by a charged membrane anchor sequence.