Tibet plateau probiotic mitigates chromate toxicity in mice by alleviating oxidative stress in gut microbiota
Tibet plateau probiotic mitigates chromate toxicity in mice by alleviating oxidative stress in gut microbiota
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西藏高原益生菌通过减轻肠道微生物群的氧化应激来减轻小鼠铬酸盐毒性
DOI：
10.1038/s42003-020-0968-3
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发表时间：
2020-05-15
影响因子：
5.9
通讯作者：
Li, Xiangkai
中科院分区：
文献类型：
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作者：
Feng, Pengya;Ye, Ze;Li, Xiangkai
Feng等人表明，用乳酸片球菌菌株BT36定殖小鼠减少了肝脏中的铬酸盐积累，这与肠道微生物群中Cr（VI）抗性基因表达的变化有关。本研究表明，BT36调节肠道菌群对Chrome暴露的反应，保护小鼠免受铬酸盐毒性。益生菌可以保护动物和人类免受重金属的侵害，但其解毒机制尚未完全阐明。在这里，小鼠补充了从西藏高原酸奶中分离的乳酸片球菌菌株BT36，具有很强的抗氧化活性，但没有铬酸盐还原能力，持续20天，以确保肠道定殖。菌株BT36降低了小鼠肝脏中的铬酸盐积累，减少了氧化应激，并减轻了组织学损伤。粪便微生物群的16S rRNA和元转录组测序分析表明，BT36逆转了Cr（VI）诱导的肠道微生物组成和代谢活性的变化。具体而言，BT36恢复了788个基因的表达，包括34个固有的铬补救相关基因。对BT36调控的10个未注释基因的功能分析表明，在肠道微生物群中存在一个新的Cr（VI）还原基因。因此，BT36可以调节肠道微生物群以响应Cr（VI）诱导的氧化应激并保护免受Cr毒性。
Feng et al. show that colonization of mice with Pediococcus acidilactici strain BT36 reduces chromate accumulation in the liver, which is associated with changes in expression of a Cr(VI)-resistance gene in the gut microbiota. This study suggests that BT36 regulates the gut microbiota in response to chrome exposure, protecting mice against chromate toxicity.Heavy metal contamination in food endangers human health. Probiotics can protect animals and human against heavy metals, but the detoxification mechanism has not been fully clarified. Here, mice were supplemented with Pediococcus acidilactici strain BT36 isolated from Tibetan plateau yogurt, with strong antioxidant activity but no chromate reduction ability for 20 days to ensure gut colonization. Strain BT36 decreased chromate accumulation, reduced oxidative stress, and attenuated histological damage in the liver of mice. 16S rRNA and metatranscriptome sequencing analysis of fecal microbiota showed that BT36 reversed Cr(VI)-induced changes in gut microbial composition and metabolic activity. Specifically, BT36 recovered the expressions of 788 genes, including 34 inherent Cr remediation-relevant genes. Functional analysis of 10 unannotated genes regulated by BT36 suggested the existence of a new Cr(VI)-reduction gene in the gut microbiota. Thus, BT36 can modulate the gut microbiota in response to Cr(VI) induced oxidative stress and protect against Cr toxicity.