ALTERNATIVE SPLICING GENERATES FUNCTIONALLY DISTINCT N-METHYL-D-ASPARTATE RECEPTORS
ALTERNATIVE SPLICING GENERATES FUNCTIONALLY DISTINCT N-METHYL-D-ASPARTATE RECEPTORS
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DOI：
10.1073/pnas.89.18.8552
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发表时间：
1992-09-15
影响因子：
11.1
通讯作者：
SHNEIDER, NA
中科院分区：
文献类型：
--
作者：
NAKANISHI, N;AXEL, R;SHNEIDER, NA
我们已经使用表达克隆在非洲爪蟾卵母细胞分离两个不同的cDNA编码功能N-甲基-D-天冬氨酸（NMDA）受体亚基。这两种受体（NMDA-R1 A和-R1 B）显示不同的药理学特性，作为一个结果的替代外显子添加在推定的配体结合结构域。剪接选择受到调节，使得R1 B是小脑中受体的主要形式，而R1 A在其他脑区域中占主导地位。在卵母细胞中单独表达任一亚基导致NMDA诱发的内向电流，其电生理特性与在神经元中观察到的NMDA受体的电生理特性非常相似。因此，神经元中NMDA受体表现出的复杂性质可以通过单个受体亚基的表达产生。
We have used expression cloning in Xenopus oocytes to isolate two different cDNAs encoding functional N-methyl-D-aspartate (NMDA) receptor subunits. The two receptors (NMDA-R1A and -R1B) display different pharmacologic properties as a consequence of alternative exon addition within the putative ligand-binding domain. The splicing choice is regulated such that R1B is the predominant form of receptor in the cerebellum, whereas R1A predominates in other brain regions. Expression of either of the subunits alone in oocytes results in an NMDA-evoked inward current with electrophysiologic properties closely resembling those of the NMDA receptors observed in neurons. Thus, the complex properties exhibited by the NMDA receptor in neurons can be generated by the expression of a single receptor subunit.