Use of DNA restriction fragment length polymorphisms to document marrow engraftment and mixed hematopoietic chimerism following bone marrow transplantation.
Use of DNA restriction fragment length polymorphisms to document marrow engraftment and mixed hematopoietic chimerism following bone marrow transplantation.
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使用 DNA 限制性片段长度多态性记录骨髓移植后的骨髓植入和混合造血嵌合。
DOI：
10.1097/00007890-198703000-00016
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发表时间：
1987
期刊：
影响因子：
6.2
通讯作者：
Blume,KG
中科院分区：
文献类型：
--
作者：
Yam,PY;Petz,LD;Knowlton,RG;Wallace,RB;Stock,AD;deLange,G;Brown,VA;Donis-Keller,H;Blume,KG
我们研究了用DNA限制性片段长度多态性（RFLP）研究27例同种异体骨髓移植后骨髓移植的可行性，并将这些结果与使用红细胞抗原、细胞遗传学和免疫球蛋白同种异体获得的结果进行了比较。使用高度多态DNA探针，我们已经记录了稳定的慢性混合造血嵌合体，已经确定了短暂的混合嵌合体，即使在没有移植前DNA样本的情况下，也在回顾性研究中排除了高概率的混合嵌合体，已经记录了移植后早期的骨髓移植，并研究了复发性疾病患者白血病细胞的起源。我们已经评估了几种遗传标记的优点和缺点，并对混合嵌合患者的预后进行了初步的陈述。我们得出结论，DNA RFLP在骨髓移植研究中是一种强大而实用的遗传标记，值得进一步研究混合造血嵌合。
We have studied the feasibility of using DNA restriction fragment-length polymorphisms (RFLP) to study marrow engraftment in 27 patients after allogeneic bone marrow transplantation, and have compared these results with those obtained using red blood cell antigens, cytogenetics, and immunoglobulin allotypes. Using highly polymorphic DNA probes, we have documented stable chronic mixed hematopoietic chimerism, have identified transient mixed chimeras, have excluded mixed chimerism with high probability in retrospective studies even when a pretransplant DNA sample was not available, have documented marrow engraftment in the early posttransplant period, and have studied the origin of leukemic cells in patients with recurrent disease. We have evaluated the advantages and disadvantages of several genetic markers and have developed tentative statements concerning the prognosis of patients with mixed chimerism. We conclude that DNA RFLP are powerful and practical genetic markers in bone marrow transplantation studies and that further studies of mixed hematopoietic chimerism are warranted.