Thousandfold signal increase using field-amplified sample stacking for on-chip electrophoresis
Thousandfold signal increase using field-amplified sample stacking for on-chip electrophoresis
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DOI：
10.1002/elps.200305611
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发表时间：
2003-10-01
期刊：
影响因子：
2.9
通讯作者：
Santiago, JG
中科院分区：
文献类型：
--
作者：
Jung, B;Bharadwaj, R;Santiago, JG
场放大样品堆积（FASS）利用一定体积的注入样品和背景缓冲液之间的电导率梯度来增加样品浓度。将FASS应用于芯片上测定的主要挑战是在注入样品体积的区域中初始设置高电导率梯度边界。我们已经设计，制造和表征了一种新型的FASS-毛细管电泳（CE）芯片设计，该芯片设计使用光引发的多孔聚合物结构，以促进高梯度FASS的样品注射和流量控制。该聚合物结构提供了允许样品离子电迁移的高流阻区域。我们已经证明了一个电泳信号增加了1100倍的荧光素和Bodipy的电泳分离，分别为2 μ M和1 μ M的初始浓度。
Field-amplified sample stacking (FASS) leverages conductivity gradients between a volume of injected sample and the background buffer to increase sample concentration. A major challenge in applying FASS to on-chip assays is the initial setup of high-conductivity gradient boundaries in the region of the injected sample volume. We have designed, fabricated, and characterized a novel FASS-capillary electrophoresis (CE) chip design that uses a photoinitiated porous polymer structure to facilitate sample injection and flow control for high-gradient FASS. This polymer structure provides a region of high flow resistance that allows the electromigration of sample ions. We have demonstrated an electropherogram signal increase by a factor of 1100 in electrophoretic separations of fluorescein and Bodipy with, respectively, 2 muM and 1 muM initial concentrations.