Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus.
Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus.
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全基因组分析揭示了乙酰杆菌遗传性遗传的遗传不稳定性。
DOI：
10.1093/nar/gkp612
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发表时间：
2009-09
影响因子：
14.9
通讯作者：
Shirai M
中科院分区：
文献类型：
--
作者：
Azuma Y;Hosoyama A;Matsutani M;Furuya N;Horikawa H;Harada T;Hirakawa H;Kuhara S;Matsushita K;Fujita N;Shirai M
醋杆菌属物种已被用于酿造传统醋，并且已知具有遗传不稳定性。为了阐明其突变性，对形成多表型细胞复合体的巴氏醋杆菌 NBRC 3283 进行了基因组 DNA 测序。基因组分析显示，在由一条 2.9 Mb 染色体和 6 个质粒组成的基因组中，有超过 280 个转座子和 5 个具有超突变串联重复的基因作为共同特征。细胞复合物的 32 个分离株中存在 3 个单核苷酸突变和 5 个转座子插入。巴氏放线菌超突变性用于培育在无法存活的高温（42°C）下生长的耐温菌株。通过突变作图分析了表现出耐热性的可遗传突变体的基因组 DNA 序列，表明在适应过程中基因组中发生了 92 kb 的缺失和三个单核苷酸突变。包括巴氏杆菌在内的α-变形菌由许多细胞内共生体和寄生虫组成，它们的基因组显示出更高的进化速度和密集的基因组减少。然而，巴氏杆菌被认为是一种自由生活的细菌，它可能有潜力进化以适应季节性水果和花卉的自然生态位，与其他生物体（例如酵母和乳酸菌）一起生存。
Acetobacter species have been used for brewing traditional vinegar and are known to have genetic instability. To clarify the mutability, Acetobacter pasteurianus NBRC 3283, which forms a multi-phenotype cell complex, was subjected to genome DNA sequencing. The genome analysis revealed that there are more than 280 transposons and five genes with hyper-mutable tandem repeats as common features in the genome consisting of a 2.9-Mb chromosome and six plasmids. There were three single nucleotide mutations and five transposon insertions in 32 isolates from the cell complex. The A. pasteurianus hyper-mutability was applied for breeding a temperature-resistant strain grown at an unviable high-temperature (42°C). The genomic DNA sequence of a heritable mutant showing temperature resistance was analyzed by mutation mapping, illustrating that a 92-kb deletion and three single nucleotide mutations occurred in the genome during the adaptation. Alpha-proteobacteria including A. pasteurianus consists of many intracellular symbionts and parasites, and their genomes show increased evolution rates and intensive genome reduction. However, A. pasteurianus is assumed to be a free-living bacterium, it may have the potentiality to evolve to fit in natural niches of seasonal fruits and flowers with other organisms, such as yeasts and lactic acid bacteria.
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DOI：
10.1073/pnas.0611553104
发表时间：
2007-05-08
影响因子：
11.1
作者：
Cho, Nam-Hyuk;Kim, Hang-Rae;Kim, Ik-Sang
通讯作者：
Kim, Ik-Sang
影响因子：
4.8
作者：
Delagoutte, Emmanuelle;Goellner, Geoffrey M.;McMurray, Cynthia T.
通讯作者：
McMurray, Cynthia T.
影响因子：
3.2
作者：
Chagneau, C;Heyde, M;Laloi, P
通讯作者：
Laloi, P
影响因子：
3.2
作者：
COUCHERON, DH
通讯作者：
COUCHERON, DH
DOI：
10.1073/pnas.88.16.7160
发表时间：
1991-08-01
影响因子：
11.1
作者：
DRAKE, JW
通讯作者：
DRAKE, JW