New assay for the measurement of selenoprotein P as a sepsis biomarker from serum
New assay for the measurement of selenoprotein P as a sepsis biomarker from serum
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DOI：
10.1016/j.jtemb.2007.11.003
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发表时间：
2008-01-01
影响因子：
3.5
通讯作者：
Schomburg, Lutz
中科院分区：
文献类型：
--
作者：
Hollenbach, Birgit;Morgenthaler, Nils G.;Schomburg, Lutz
硒（Se）作为第21种蛋白质原氨基酸硒半胱氨酸被纳入硒蛋白中。危重期血清硒浓度下降，预示预后不良。血清硒主要存在于肝源性硒蛋白P （SePP）中，SePP控制抗氧化硒蛋白的表达。在这里，我们描述了一种使用两种多克隆绵羊抗人SePP抗体的免疫荧光夹心法的发展。在评估分析物的稳定性后，我们测定了健康个体和脓毒症患者样本中的SePP浓度。分析检出限为0.016 mg SePP/L血清。稀释度呈线性关系。SePP在室温下在血清中稳定至少24 h，并耐6次冻融循环。健康人血清SePP中位浓度为3.04mg SePP/L(25 -75百分位数，2.6-3.4mg/L)，对应血清硒含量为98.4 μ g/L。实验室间CV为0.06 mg/L。这与性别无关，但浓度在年轻人和老年人之间有所不同。中位SePP浓度显著(P
Selenium (Se) is incorporated into selenoproteins as the 21st proteinogenic amino acid selenocysteine. Serum Se concentrations decline during critical illness and are indicative of poor prognosis. Serum Se is mainly contained in the hepatically derived selenoprotein P (SePP) which controls the expression of antioxidative selenoproteins. Here, we describe the development of an immunoluminometric sandwich assay that uses two polyclonal sheep antihuman SePP antibodies. After assessing the stability of the analyte, we determined SePP concentrations in samples from healthy individuals and patients with sepsis. The analytical detection limit was 0.016 mg SePP/L serum. The assay was linear on dilution. SePP was stable in serum at room temperature for at least 24 h and resistant to six freeze-thaw cycles. Median SePP concentration in healthy individuals was 3.04mg SePP/L serum (25th-75th percentiles, 2.6-3.4mg/L) which corresponded to 98.4 mu g Se/L serum. The interlaboratory CV was 0.06 mg/L. There was no association with gender, but concentrations differed between young and older individuals. Median SePP concentrations were significantly (P