Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman spectroscopy
Real-time in vivo diagnosis of laryngeal carcinoma with rapid fiber-optic Raman spectroscopy
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DOI：
10.1364/boe.7.003705
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发表时间：
2016-09-01
影响因子：
3.4
通讯作者：
Huang, Zhiwei
中科院分区：
文献类型：
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作者：
Lin, Kan;Zheng, Wei;Huang, Zhiwei
我们评估了一种独特的同时指纹(FP)(即8001800厘米(-1))和高波数(HW)(即2800-3600厘米(-1))光纤拉曼光谱在内窥镜下活体诊断喉癌中的临床应用。从60例患者(正常44例，癌症16例)的101个组织部位(正常=71个；癌=30个)获得了2124个高质量的在体FP/HW拉曼光谱(正常=1321；癌=581)。正常喉组织和喉癌组织的FP/HW拉曼光谱明显不同，这可能是由于喉组织中蛋白质、脂类、核酸和结合水含量的变化所致。对获得的活体FP/HW组织拉曼光谱进行偏最小二乘判别分析和留一组织点交叉验证，对喉癌的诊断准确率为91.1%(敏感度：93.3%(28/30)；特异度：90.1%(/71))，优于FP(准确度：86.1%；敏感度：86.7%(26/30)；特异度：85.9%(61/71))或HW(准确度：84.2%；灵敏度：76.7%(23/30)；特异度：87.3%(62/71)。进一步的接收器工作特性分析再次证实了FP/HW同时拉曼技术在喉癌诊断中的最佳性能。我们首次证明了同时使用FP/HW拉曼光谱技术可以提高在内窥镜检查中对喉癌的实时在体诊断。(C)2016年度美国光学学会
We assess the clinical utility of a unique simultaneous fingerprint (FP) (i.e., 8001800 cm(-1)) and high-wavenumber (HW) (i.e., 2800-3600 cm(-1)) fiber-optic Raman spectroscopy for in vivo diagnosis of laryngeal cancer at endoscopy. A total of 2124 high-quality in vivo FP/HW Raman spectra (normal = 1321; cancer = 581) were acquired from 101 tissue sites (normal = 71; cancer = 30) of 60 patients (normal = 44; cancer = 16) undergoing routine endoscopic examination. FP/HW Raman spectra differ significantly between normal and cancerous laryngeal tissue that could be attributed to changes of proteins, lipids, nucleic acids, and the bound water content in the larynx. Partial least squares-discriminant analysis and leave-one tissue site-out, cross-validation were employed on the in vivo FP/HW tissue Raman spectra acquired, yielding a diagnostic accuracy of 91.1% (sensitivity: 93.3% (28/30); specificity: 90.1% (64/71)) for laryngeal cancer identification, which is superior to using either FP (accuracy: 86.1%; sensitivity: 86.7% (26/30); specificity: 85.9% (61/71)) or HW (accuracy: 84.2%; sensitivity: 76.7% (23/30); specificity: 87.3% (62/71)) Raman technique alone. Further receiver operating characteristic analysis reconfirms the best performance of the simultaneous FP/HW Raman technique for laryngeal cancer diagnosis. We demonstrate for the first time that the simultaneous FP/HW Raman spectroscopy technique can be used for improving real-time in vivo diagnosis of laryngeal carcinoma during endoscopic examination. (C) 2016 Optical Society of America