DELETION ANALYSIS OF CIS-ACTING AND TRANS-ACTING ELEMENTS INVOLVED IN REPLICATION OF ALFALFA MOSAIC-VIRUS RNA-3 INVIVO
DELETION ANALYSIS OF CIS-ACTING AND TRANS-ACTING ELEMENTS INVOLVED IN REPLICATION OF ALFALFA MOSAIC-VIRUS RNA-3 INVIVO
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DOI：
10.1016/0042-6822(91)90997-p
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发表时间：
1991-08-01
期刊：
影响因子：
3.7
通讯作者：
BOL, JF
中科院分区：
文献类型：
--
作者：
VANDERKUYL, AC;NEELEMAN, L;BOL, JF
将苜蓿花叶病毒（AIMV）RNA 3的DNA拷贝在体外转录成编码和非编码序列缺失的RNA分子。在来自表达AIMV RNA 1和2的DNA拷贝的转基因烟草植物的原生质体中研究了这些转录物的复制。5 ′端P3基因的缺失（编码假定的病毒转运功能）不影响复制，而3′端外壳蛋白基因的缺失使RNA 3的复制减少约100倍。在原生质体中的RNA 4，外壳蛋白信使的合成所需的序列，更广泛的亚基因组启动子，其特征在于以前在anin vitroreplicase测定。在RNA 3的5′端，169个核苷酸的序列足以复制，而112个核苷酸的序列则不足以复制。3′-末端缺失多达133个核苷酸将复制降低到低但显著的水平。进一步的3′-缺失消除了复制。
DNA copies of alfalfa mosaic virus (AIMV) RNA 3 were transcribedin vitrointo RNA molecules with deletions in coding and noncoding sequences. The replication of these transcripts was studied in protoplasts from transgenic tobacco plants expressing DNA copies of AIMV RNAs 1 and 2. Deletions in the5′-proximal P3 gene, encoding the putative viral transport function, did not affect replication whereas deletions in the 3′-proximal coat protein gene reduced replication of RNA 3 by about 100-fold. Sequences required for the synthesis in protoplasts of RNA 4, the coat protein messenger, were more extensive than the subgenomic promoter characterized previously in anin vitroreplicase assay. At the 5′-end of RNA 3 a sequence of 169 nucleotides was sufficient for replication whereas a sequence of 112 nucleotides was not. 3′-Terminal deletions up to 133 nucleotides reduced replication to a low but significant level. Further 3′-deletions abolished replication.