Curcumin Inhibits the Tumorigenesis of Breast Cancer by Blocking Tafazzin/Yes-Associated Protein Axis (Retracted article. See vol. 12, pg. 5739, 2020)
Curcumin Inhibits the Tumorigenesis of Breast Cancer by Blocking Tafazzin/Yes-Associated Protein Axis (Retracted article. See vol. 12, pg. 5739, 2020)
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DOI：
10.2147/cmar.s246691
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发表时间：
2020-01-01
影响因子：
3.3
通讯作者：
Yuan, Hongyan
中科院分区：
文献类型：
--
作者：
Shen, Yuxiu;Han, Zaigang;Yuan, Hongyan
目的：本研究旨在探讨姜黄素对乳腺癌（BC）的抗肿瘤作用及其涉及Tafazzin（TAZ）/Yes相关蛋白（YAP）轴的潜在机制。方法：用不同浓度的姜黄素（0、10、20和30μM）处理BC细胞（MCF-7和MDA-MB-231细胞）。分别通过MTT、集落形成、流式细胞术、伤口愈合和Transwell实验检测BC细胞的活力、集落形成、凋亡、迁移和侵袭。 Western blot检测Hippo信号通路效应子TAZ和YAP蛋白表达。将MDA-MB-231细胞注射到小鼠体内，验证姜黄素的体内抗肿瘤作用。结果：姜黄素（20和30μM）抑制MCF-7和MDA-MB-231细胞的增殖、迁移和侵袭，并促进细胞凋亡。姜黄素降低 MCF-7 和 MDA-MB-231 细胞中 TAZ 和 YAP 的蛋白表达。 YAP 的过表达逆转了姜黄素对 MDA-MB-231 细胞的抗肿瘤作用。此外，姜黄素（100、200和300 mg/kg/d）抑制小鼠肿瘤异种移植物的生长，并下调肿瘤异种移植物中TAZ和YAP的蛋白表达。而300 mg/kg/d浓度的姜黄素则减缓了小鼠体重的增加。结论：姜黄素通过阻断TAZ/YAP轴抑制BC的肿瘤发生。
Purpose: This study was aimed to explore the anti-tumor effect of curcumin on breast cancer (BC) and the underlying mechanism involving Tafazzin (TAZ)/Yes-associated protein (YAP) axis.Methods: Different concentrations of curcumin (0, 10, 20 and 30 mu M) were used to treat BC cells (MCF-7 and MDA-MB-231 cells). The viability, colony formation, apoptosis, migration, and invasion of BC cells were detected by MTT, colony formation, flow cytometry, wound-healing and transwell assay, respectively. The protein expression of TAZ and YAP (effectors of Hippo signaling pathway) was detected by Western blot. MDA-MB-231 cells were injected into mice to verify the anti-tumor effect of curcumin in vivo.Results: Curcumin (20 and 30 mu M) inhibited the proliferation, migration and invasion, and promoted the apoptosis of MCF-7 and MDA-MB-231 cells. Curcumin decreased the protein expression of TAZ and YAP in MCF-7 and MDA-MB-231 cells. Overexpression of YAP reversed the anti-tumor effect of curcumin on MDA-MB-231 cells. In addition, curcumin (100, 200 and 300 mg/kg/d) inhibited the growth of tumor xenografts in mice, and down-regulated the protein expression of TAZ and YAP in tumor xenografts. However, curcumin at a concentration of 300 mg/kg/d slowed the increasing of body weight in mice.Conclusion: Curcumin inhibited the tumorigenesis of BC by blocking TAZ/YAP axis.