Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia
Altered microRNA profiles of testicular biopsies from patients with nonobstructive azoospermia
复制标题
非梗阻性无精症患者睾丸活检的 microRNA 谱发生改变。
DOI：
10.4103/aja.aja_35_19
复制
发表时间：
2020-01-01
影响因子：
2.9
通讯作者：
Jiang, Hui
中科院分区：
文献类型：
--
作者：
Zhang, Hai-Tao;Zhang, Zhe;Jiang, Hui
许多研究表明 microRNA (miRNA) 在精子发生过程中发挥着至关重要的作用。然而，对于非梗阻性无精症 (NOA) 中睾丸组织 miRNA 谱的改变知之甚少。使用微阵列技术，确定了 NOA 患者睾丸活检和正常睾丸组织的 miRNA 表达谱。进行生物信息学分析来预测已识别 miRNA 的丰富生物过程和功能。通过定量逆转录酶聚合酶链反应（qRT-PCR）验证微阵列数据，然后用更大的样本量验证其结果。分析 miRNA 表达水平与临床特征之间的相关性。采用受试者工作特征（ROC）曲线分析评估miRNA对无精症的诊断能力。层次聚类显示，NOA 组和对照组之间有 129 个 miRNA 表达存在显着差异。生物信息学分析表明差异表达的miRNA参与精子发生、细胞周期和有丝分裂前期。在随后的qRT-PCR检测中，所选的miRNA表达水平与微阵列结果一致，并且在更大的样本量下获得了相似的验证结果。一些临床特征与某些 miRNA 的表达显着相关。特别是，我们鉴定了两种 miRNA（miR-10b-3p 和 miR-34b-5p）的组合，可以作为无精症的预测生物标志物。这项研究提供了 NOA 患者睾丸活检中改变的 miRNA 谱，并检查了 miRNA 在精子发生中的作用。这些特征可能有助于预测和诊断无精子症患者睾丸精子的存在。
Many studies have shown that microRNAs (miRNAs) play vital roles during the spermatogenesis. However, little is known about the altered miRNA profiles of testicular tissues in nonobstructive azoospermia (NOA). Using microarray technology, the miRNA expression profiles of testicular biopsies from patients with NOA and of normal testicular tissues were determined. Bioinformatics analyses were conducted to predict the enriched biological processes and functions of identified miRNAs. The microarray data were validated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), the results of which were then validated with a larger sample size. Correlations between the miRNA expression levels and clinical characteristics were analyzed. Receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic ability of miRNAs for azoospermia. Hierarchical clustering showed that 129 miRNAs were significantly differentially expressed between the NOA and control groups. Bioinformatics analysis indicated that the differentially expressed miRNAs were involved in spermatogenesis, cell cycle, and mitotic prometaphase. In the subsequent qRT-PCR assays, the selected miRNA expression levels were consistent with the microarray results, and similar validated results were obtained with a larger sample size. Some clinical characteristics were significantly associated with the expression of certain miRNAs. In particular, we identified a combination of two miRNAs (miR-10b-3p and miR-34b-5p) that could serve as a predictive biomarker of azoospermia. This study provides altered miRNA profiles of testicular biopsies from NOA patients and examines the roles of miRNAs in spermatogenesis. These profiles may be useful for predicting and diagnosing the presence of testicular sperm in individuals with azoospermia.