Nestin expression as a new marker in malignant peripheral nerve sheath tumors
Nestin expression as a new marker in malignant peripheral nerve sheath tumors
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DOI：
10.1111/j.1440-1827.2006.02059.x
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发表时间：
2007-02-01
影响因子：
2.2
通讯作者：
Illei, Peter B.
中科院分区：
文献类型：
--
作者：
Shimada, Satoko;Tsuzuki, Toyonori;Illei, Peter B.
恶性周围神经鞘瘤（MPNST）可能很难诊断，因为它缺乏特异性免疫组化标记。S-100是MPNST的有用标志物，但其诊断价值有限。最近的研究表明，巢蛋白，这是一种中间丝蛋白，在神经外胚层干细胞中表达。对35例MPNST和其他梭形细胞肿瘤中巢蛋白和其他三种神经标志物（S-100、CD 56和蛋白基因产物9.5（PGP 9.5））的免疫组织化学诊断价值进行了评价。所有MPNST病例nestin均呈强阳性表达，并有胞浆染色。S-100、CD 56和PGP 9.5染色阳性的病例较少（分别为17/35、11/35和29/35例），且染色范围较小。巢蛋白在10例平滑肌瘤中呈阴性表达，在10例神经鞘瘤、3例神经纤维瘤、2例滑膜肉瘤、2例脂肪肉瘤、4例癌性肉瘤和3例恶性纤维组织细胞瘤中呈弱阳性表达。相反，在10/10的横纹肌肉瘤、15/19的平滑肌肉瘤和9/9的促结缔组织增生性黑色素瘤中观察到强巢蛋白阳性。Nestin对MPNST的诊断敏感性高于其他神经标志物，联合应用Nestin免疫组化染色可作为MPNST的诊断指标。
Malignant peripheral nerve sheath tumor (MPNST) can be difficult to diagnose because it lacks specific immunohistochemical markers. S-100, which is a useful marker of MPNST, has limited diagnostic utility. Recent studies suggest that nestin, which is an intermediate filament protein, is expressed in neuroectodermal stem cells. The diagnostic utility of immunostains for nestin and three other neural markers (S-100, CD56 and protein gene product 9.5 (PGP 9.5)) were evaluated in 35 cases of MPNST and in other spindle cell tumors. All MPNST cases were strongly positive for nestin and had cytoplasmic staining. Stains for S-100, CD56, and PGP 9.5 were positive in fewer cases (17/35, 11/35, and 29/35 cases, respectively), and had less extensive staining. Nestin was negative in 10/10 leiomyomas, and weak nestin expression was seen in 10/10 schwannomas, 3/10 neurofibromas, 2/8 synovial sarcomas, 2/10 liposarcomas, 4/7 carcinosarcomas and 3/7 malignant fibrous histiocytomas. In contrast, strong nestin positivity was seen in 10/10 rhabdomyosarcomas, 15/19 leiomyosarcomas, and 9/9 desmoplastic melanomas. Nestin is more sensitive for MPNST than other neural markers and immunostains for nestin in combination with other markers could be useful in the diagnosis of MPNST.