ESTABLISHMENT AND CHARACTERIZATION OF HUMAN T-HYBRID CELLS SECRETING IMMUNOREGULATORY MOLECULES
ESTABLISHMENT AND CHARACTERIZATION OF HUMAN T-HYBRID CELLS SECRETING IMMUNOREGULATORY MOLECULES
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DOI：
10.1073/pnas.78.12.7717
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
KISHIMOTO, T
中科院分区：
文献类型：
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作者：
OKADA, M;YOSHIMURA, N;KISHIMOTO, T
人T细胞与抗氮鸟嘌呤的人T细胞系杂交产生分泌几种免疫调节分子的T杂交细胞系。核型分析，HLA表型和其他表面表型，如T细胞特异性抗原或受体的绵羊红细胞和有丝分裂原反应的模式，证实了次黄嘌呤/氨基蝶呤/胸腺嘧啶耐药细胞系是人类T-T杂交瘤。建立的杂交克隆之一（24-A）分泌人白细胞介素-2（IL-2）。培养物上清液诱导伴刀豆球蛋白A刺激的鼠T细胞的增殖，并支持IL-2依赖性人细胞毒性T细胞系的增殖。在培养上清液中未显示任何IL-2活性的克隆（38-B）中，在植物血凝素存在下，加入巨噬细胞诱导IL-2产生，表明白细胞介素-I诱导T杂交细胞中的IL-2产生。建立了分泌杀伤辅助因子的杂交细胞。来自该克隆55-A的培养物上清液有助于诱导针对UV处理的人B-胚样细胞的细胞毒性T细胞，但未显示任何IL-2活性。
Hybridization of human T cells with an azaguanine-resistant human T cell line gave rise to T hybrid cell lines secreting several immunoregulatory molecules. Analyses of karyotypes, HLA phenotypes and other surface phenotypes, such as T-cell-specific antigens or receptors for sheep erythrocytes and the patterns of mitogen responsiveness confirmed that the hypoxanthine/aminopterin/thymidine-resistant cell lines were human T-T hybridomas. One of the established hybrid clones (24-A) secreted human interleukin-2 (IL-2). The culture supernatants induced the proliferation of concanavalin A-stimulated murine T cells and supported the proliferation of an IL-2-dependent human cytotoxic T-cell line. In a clone (38-B) that did not show any IL-2 activity in culture supernatants, the addition of macrophages induced IL-2 production in the presence of phytohemagglutinin, suggesting that interleukin-I induced IL-2 production in T hybrid cells. Hybrid cells secreting killer helper factor were established. The culture supernatants from this clone, 55-A, helped the induction of cytotoxic T cells against UV-treated human B-blastoid cells but did not show any IL-2 activity.