A DIPLOID EPITHELIAL-CELL LINE FROM NORMAL ADULT-RAT LIVER WITH PHENOTYPIC PROPERTIES OF OVAL CELLS
A DIPLOID EPITHELIAL-CELL LINE FROM NORMAL ADULT-RAT LIVER WITH PHENOTYPIC PROPERTIES OF OVAL CELLS
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DOI：
10.1016/0014-4827(84)90666-9
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发表时间：
1984-01-01
影响因子：
3.7
通讯作者：
GRISHAM, JW
中科院分区：
文献类型：
--
作者：
TSAO, MS;SMITH, JD;GRISHAM, JW
从成年雄性Fischer-344大鼠的肝脏中分离出二倍体上皮细胞系（称为WB-F344），并研究了细胞的表型特征。这些单元格测量. apprx。新鲜分离肝细胞体积的2/5。它们在组织化学上对G-6-P呈阴性，而对γ-谷氨酰胺呈弱阳性。谷氨酰转肽酶它们产生广泛的细胞间网硬蛋白纤维，其对纤连蛋白进行免疫细胞化学染色，并且它们合成α-纤维素和α-纤维素。甲胎蛋白和白蛋白，但他们不积累糖原颗粒。超微结构上，它们是多边形细胞，有许多细胞间桥粒和连接点，它们部分被基底膜样物质包围。细胞质细胞器包括少量但有时扩张的粗面内质网、溶酶体、丰富的游离核糖体、稀疏的滑面内质网和高尔基膜、微体和小型多形线粒体。它们表达醛缩酶的A和C同工酶、丙酮酸激酶的K同工酶、乳酸脱氢酶的LDH[乳酸脱氢酶]2至LDH 5同工酶和胎肝型碱性磷酸酶同工酶。当与分离和纯化的正常肝细胞，胆管上皮（导管）细胞和卵圆细胞的表型相比，从用化学致癌物处理的肝脏中分离的肝上皮细胞系的表型特性最类似于卵圆细胞。
A diploid epithelial cell line (termed WB-F344) was isolated from the liver of an adult male Fischer-344 rat and the phenotypic characteristics of the cells were studied. These cells measure .apprx. 2/5 the volume of freshly isolated hepatocytes. They are histochemically negative for G-6-P and weakly positive for .gamma.-glutamyl transpeptidase. They produce extensive intercellular reticulin fibers which stain immunocytochemically for fibronectin, and they synthesize both .alpha.-fetoprotein and albumin, but they do not accumulate glycogen particles. Ultrastructurally, they are polygonal cells with numerous intercellular desmosomes and nexus junctions, and they are partially surrounded by basement membrane-like material. Cytoplasmic organelles include few, but sometimes dilated profiles of rough endoplasmic reticulum, lysosomes, abundant free ribosomes, sparse smooth endoplasmic reticulum and Golgi membranes, microbodies and small, pleomorphic mitochondria. They express A and C isozymes of aldolase, K isozyme of pyruvate kinase, LDH[lactate dehydrogenase]2 to LDH5 isozymes of lactate dehydrogenase, and fetal liver-type alkaline phosphatase isozyme. When compared with the phenotypes of isolated and purified normal hepatocytes, biliary epithelial (ductular) cells and oval cells isolated from livers treated with chemical carcinogens, the phenotypic properties of the liver epithelial cell line in culture most resemble those of the oval cells.