Competitive adsorption of human serum albumin and gamma-globulin from a binary protein mixture onto hexadecyltrichlorosilane coated glass.
Competitive adsorption of human serum albumin and gamma-globulin from a binary protein mixture onto hexadecyltrichlorosilane coated glass.
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将二元蛋白质混合物中的人血清白蛋白和丙种球蛋白竞争性吸附到十六烷基三氯硅烷涂层玻璃上。
DOI：
10.1163/156856298x00488
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
M. Foster
中科院分区：
文献类型：
--
作者：
Y. S. Tremsina;V. Sevastianov;S. Petrash;W. Dando;M. Foster
用全内反射荧光（TIRF）技术研究了十六烷基三氯硅烷涂层玻璃（HTS-glass）从异硫氰酸荧光素（FITC）标记的人血清白蛋白（HSA-FITC）和γ-球蛋白（HGG-FITC）模型溶液中竞争吸附蛋白质的动力学。HGG置换HSA-FITC的过程与吸附在玻璃上的HSA的构象状态无关。在HTS-玻璃上，蛋白质的置换受到大量CH 3-封端的烷基尾的存在的阻碍，所述烷基尾诱导从简单溶液中吸附的HSA-FITC和HGG-FITC的构象（重定向）变化。与HSA相反，HGG从简单溶液吸附到HTS-玻璃上的特征在于在动力学曲线的初始部分中不存在不可逆吸附。HSA和HGG-FITC之间的竞争诱导HTS-玻璃表面上末端吸附的HGG-FITC的替换，随后HGG-FITC解吸到溶液中。当溶液中HSA浓度进一步增加时，HSA对吸附位点的竞争占上风，这导致吸附的HGG-FITC的量减少，因此，其置换速率降低。
The kinetics of competitive adsorption of proteins onto hexadecyltrichlorosilane coated glass (HTS-glass) from model solutions containing fluorescein isothiocyanate (FITC)-labeled human serum albumin (HSA-FITC) and gamma-globulin (HGG-FITC) were studied by total internal reflection fluorescence (TIRF) spectroscopy. The processes of displacement of HSA-FITC by HGG are independent of the conformational state of HSA adsorbed onto glass. On HTS-glass, displacement of protein is hindered by the presence of large numbers of CH3-terminated alkyl tails which induce conformational (reorientational) changes in HSA-FITC and HGG-FITC adsorbed from simple solutions. In contrast to HSA, adsorption of HGG onto HTS-glass from a simple solution is characterized by the absence of irreversible adsorption in the initial portion of the kinetic curve. Competition between HSA and HGG-FITC induces replacement of end-on adsorbed HGG-FITC on HTS-glass surface with subsequent desorption of the HGG-FITC into solution. Upon further increase in the HSA concentration in solution the competition of HSA for adsorption sites prevails, which leads to a decrease in the amount of adsorbed HGG-FITC and, consequently, to a decrease in the rate of its displacement.