A study of associations between early DHA status and fatty acid desaturase (FADS) SNP and developmental outcomes in children of obese mothers
A study of associations between early DHA status and fatty acid desaturase (FADS) SNP and developmental outcomes in children of obese mothers
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DOI：
10.1017/s0007114516004645
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发表时间：
2017-01-01
影响因子：
3.6
通讯作者：
Lauritzen, Lotte
中科院分区：
文献类型：
--
作者：
Andersen, Karina R.;Harslof, Laurine B. S.;Lauritzen, Lotte
来自饮食或内源性合成的DHA被认为会影响婴儿的发育，然而，结果并不确定。在这项研究中，我们旨在验证之前观察到的脂肪酸去饱和酶基因簇(FADS)SNP特异性与9个月大儿童红细胞DHA状态的相关性，以及性别与发育结果的相关性。这项研究是在166名肥胖母亲的孩子(55%是男孩)中进行的。在9个月龄时采集血液样本，分析红细胞脂肪酸组成，并在3岁时用年龄和阶段问卷评估发育结果。红细胞DHA水平介于44%至99%的脂肪酸之间，但与FADS、SNP或其他潜在决定因素没有任何关联。回归分析显示红细胞DHA与个人社交技能(18(95%CI 03，33)，P=0019)和解决问题(34(95%CI 12，56，P=0003)分相关。Rs1535和rs174575的小等位基因(G变异)与个人社交技能呈反向关联(P分别为0062和0068)，根据9个月龄时观察到的对红细胞DHA的影响(26(95%CI 001，51)，P=0011)，当SNP与Rs1535和rs174575合并时，这种关联变得显著。Rs174575基因在精细运动评分上存在性别-SNP交互作用(P=0016)，这是因为携带少量等位基因的女孩得分较高(P=0043)，而在男孩中则没有影响。总而言之，在这一小群肥胖母亲的孩子中，DHA增加的FADS SNP和红细胞DHA状态一直与个人社交技能的改善有关，尽管不分性别，但样本太少，无法验证潜在的性别特定影响。
DHA from diet or endogenous synthesis has been proposed to affect infant development, however, results are inconclusive. In this study, we aim to verify previously observed fatty acid desaturase gene cluster (FADS) SNP-specific associations with erythrocyte DHA status in 9-month-old children and sex-specific association with developmental outcomes. The study was performed in 166 children (55 % boys) of obese mothers. Erythrocyte fatty acid composition was analysed in blood-samples obtained at 9 months of age, and developmental outcomes assessed by the Ages and Stages Questionnaire at 3 years. Erythrocyte DHA level ranged from 44 to 99 % of fatty acids, but did not show any association with FADS SNP or other potential determinants. Regression analysis showed associations between erythrocyte DHA and scores for personal-social skills ( 18 (95 % CI 03, 33), P=0019) and problem solving ( 34 (95 % CI 12, 56), P=0003). A tendency was observed for an association in opposite direction between minor alleles (G-variant) of rs1535 and rs174575 and personal-social skills (P=0062 and 0068, respectively), which became significant when the SNP were combined based on their previously observed effect on erythrocyte DHA at 9 months of age ( 26 (95 % CI 001, 51), P=0011). Sex-SNP interaction was indicated for rs174575 genotype on fine motor scores (P=0016), due to higher scores among minor allele carrying girls (P=0043), whereas no effect was seen among boys. In conclusion, DHA-increasing FADS SNP and erythrocyte DHA status were consistently associated with improved personal-social skills in this small cohort of children of obese mothers irrespective of sex, but the sample was too small to verify potential sex-specific effects.