ENDOGENOUS MAMMARY-TUMOR VIRUS-DNA VARIES AMONG WILD MICE AND SEGREGATES DURING INBREEDING
ENDOGENOUS MAMMARY-TUMOR VIRUS-DNA VARIES AMONG WILD MICE AND SEGREGATES DURING INBREEDING
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DOI：
10.1038/278418a0
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发表时间：
1979-01-01
期刊：
影响因子：
64.8
通讯作者：
VARMUS, HE
中科院分区：
文献类型：
--
作者：
COHEN, JC;VARMUS, HE
正常小鼠内源性小鼠乳腺肿瘤病毒（MMTV）的前病毒可以通过分子杂交鉴定，并使用限制性内切酶进行区分。野生小鼠表现出显着的异质性相对于拷贝数和内源性MMTV特异性DNA的插入位点，偶尔小鼠显然没有MMTV DNA。60年前，Bagg白化病小鼠和DBA小鼠杂交后，实验室小鼠中存在的几种不同的MMTV前病毒像稳定的、独立的遗传元件一样在近亲繁殖中分离。结果表明，内源性前病毒是通过生殖细胞的多次独立感染建立的，而不是通过祖先前病毒或细胞基因的体细胞突变。
Proviruses of the mouse mammary tumor virus (MMTV) endogenous to normal mice can be identified by molecular hybridization and distinguished using restriction endonucleases. Feral mice display marked heterogeneity with respect to the number of copies and the sites of insertion of endogenous MMTV-specific DNA, with occasional mice apparently free of MMTV DNA. Several different MMTV proviruses present in laboratory mice segregated like stable, independent genetic elements during the inbreeding which followed a cross between Bagg albino and DBA mice 60 yr ago. The results indicate that endogenous proviruses were established by multiple, independent infections of germ cells rather than by somatic mutation of ancestral proviruses or of cellular genes.