Interface of biomolecular condensates modulates redox reactions
Interface of biomolecular condensates modulates redox reactions
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DOI：
10.1016/j.chempr.2023.04.001
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发表时间：
2023-06-08
期刊：
影响因子：
23.5
通讯作者：
Chilkoti, Ashutosh
中科院分区：
文献类型：
--
作者：
Dai, Yifan;Chamberlayne, Christian F.;Chilkoti, Ashutosh
生物分子凝聚物介导多种细胞过程。冷凝物形成的密度转变过程导致分子的选择性分配，这定义了冷凝物内独特的化学环境。然而，化学环境的基本特征以及这种环境有助于凝聚态功能的机制尚未被揭示。在这里，我们报告说，由于相分离引起的离子和分子的密度转变，在冷凝物和本体环境之间的液-液界面处建立了电势梯度，从而形成了电场。我们发现缩合物界面可以在体外和活细胞中驱动自发的氧化还原反应。我们的结果揭示了冷凝物界面的基本物理化学性质以及界面调节生化活性的机制。
Biomolecular condensates mediate diverse cellular processes. The density-transition process of condensate formation results in the selective partitioning of molecules, which defines a distinct chemical environment within the condensates. However, the fundamental features of the chemical environment and the mechanisms by which such an environment can contribute to condensate functions have not been revealed. Here, we report that an electric potential gradient, thereby an electric field, is established at the liquid-liquid interface between the condensate and the bulk environment as a result of the density transition of ions and molecules brought about by phase separation. We find that the interface of condensates can drive spontaneous redox reactions in vitro and in living cells. Our results uncover a fundamental physicochemical property of the interface of condensates and the mechanism by which the interface can modulate biochemical activities.