Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C
Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C
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DOI：
10.1074/jbc.m403029200
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发表时间：
2004-11-05
影响因子：
4.8
通讯作者：
Urano, T
中科院分区：
文献类型：
--
作者：
Li, XY;Sakashita, G;Urano, T
一个丝氨酸/苏氨酸激酶Aurora家族在有丝分裂事件的协调中构成关键调节因子。人类Aurora旁系同源物Aurora-A、Aurora-B和Aurora-C具有高度保守的催化结构域。对Aurora-A和Aurora-B作用的广泛研究揭示了它们在有丝分裂过程中不同的定位和功能，而对Aurora-C知之甚少。目前的研究表明，人类Aurora-C是一种染色体乘客蛋白，与Aurora-B和内着丝粒蛋白（INCENP）形成复合物，这是已知的乘客蛋白。我们表明，INCENP结合并激活Aurora-C在体内和体外。此外，Aurora-C与INCENP共表达可促进哺乳动物细胞中内源性组蛋白H3的磷酸化，即使这种磷酸化不足以在间期细胞中建立染色体凝聚。因此，我们认为Aurora-C是一种新的染色体乘客蛋白，与Aurora-B合作，调节哺乳动物细胞有丝分裂染色体动力学。
A family of serine/threonine kinase Aurora constitutes a key regulator in the orchestration of mitotic events. The human Aurora paralogues Aurora-A, Aurora-B, and Aurora-C have a highly conserved catalytic domain. Extensive studies on the role of Aurora-A and Aurora-B have revealed distinct localizations and functions in regulating mitotic processes, whereas little is known about Aurora-C. The present study shows that human Aurora-C is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere protein ( INCENP), which are known passenger proteins. We show that INCENP binds and activates Aurora-C in vivo and in vitro. Furthermore, Aurora-C co-expressed with INCENP elicits the phosphorylation of endogenous histone H3 in mammalian cells, even though this phosphorylation is not sufficient to establish chromosome condensation in interphase cells. We therefore suggest that Aurora-C is a novel chromosomal passenger protein that cooperates with Aurora-B to regulate mitotic chromosome dynamics in mammalian cells.