A droplet-based gradient microfluidic to monitor and evaluate the growth of Chlorella vulgaris under different levels of nitrogen and temperatures
A droplet-based gradient microfluidic to monitor and evaluate the growth of Chlorella vulgaris under different levels of nitrogen and temperatures
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DOI：
10.1016/j.algal.2019.101657
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发表时间：
2019-12-01
影响因子：
5.1
通讯作者：
Shafik, Hesham Mohamed
中科院分区：
文献类型：
--
作者：
Saad, Marwa Gamal;Selahi, Amirali;Shafik, Hesham Mohamed
藻类是不同工业产品的潜在来源。然而，利用藻类生产生物燃料仍然需要找到有价值的经济方法。选择藻类和优化培养条件被认为是生物燃料生产的主要挑战。传统的分批培养技术费时费力。基于PDMS的微流控技术是一种省时省力的高通量单细胞分析技术。我们提出了基于梯度的微流体作为研究不同养殖条件下藻类生长的工具。应用我们的设备提供了在200 S中产生不同浓度的介质营养液滴的机会，这保存了使用传统技术制备此类溶液的试剂和时间。将普通小球藻包裹在不同硝态氮浓度为2.46、6.69、10.91和15.14 mm/L的均匀液滴中，将分离的装置分别置于20、27和37℃的不同温度下，计算每个液滴中每个细胞的光合能转化。结果表明，硝酸根浓度越高，生长动力学越高。实验结果表明，最适硝酸盐浓度为15.14 mm/L，最适温度为37℃，是梯度芯片在藻类生物技术领域应用的第三次尝试。该方法独特，使用简单，使用效果好。
Algae are potential sources for different industrial products. However, biofuel production from algae still needs to reach valuable economical approaches. Select algae and optimize culture conditions considered main challenges for biofuel production. Traditional batch cultures' techniques are time and labor consuming. PDMS-based Microfluidics is time-and labor-saving technique with high throughput single cell analysis. We present gradient-based microfluidics as tools for studying algal growth under different cultivation conditions. Applying our device provides the opportunity to generate droplets with different concentrations of medium nutrients' in 200 s which keeps reagent and time for preparing such solutions using conventional techniques. Chlorella vulgaris was encapsulated inside uniformly droplets with different NO3--N concentrations of 2.46, 6.69, 10.91, and 15.14 mM/L. separated devices were exposed to different temperatures of 20, 27, and 37 degrees C. The photosynthetic energy conversion for each cell per droplet was calculated. It was observed that the higher the NO3- concentration, the higher the growth kinetics. According to the presented results, the optimum nitrate and temperature conditions were 15.14 mM/L and 37 degrees C. Our device is considered the third effort for applying a gradient chip in algal biotechnology field. It is unique and simple in use with high competence results.