In ovo electroporation of avian somites
In ovo electroporation of avian somites
复制标题
DOI：
10.1002/dvdy.10433
复制
发表时间：
2004-03-01
影响因子：
2.5
通讯作者：
Marcelle, C
中科院分区：
文献类型：
--
作者：
Scaal, M;Gros, J;Marcelle, C
卵内电穿孔是一种将基因转移到鸡胚神经和间质组织中的成熟方法。然而，由于技术困难，体节电穿孔一直受到效率低下的阻碍。在这里，我们提出了一种强大的电穿孔技术来电穿孔鸟类体节和亚群；卵内体细胞的高效率。我们证明了使用单个或组合的质粒表达载体对不同体细胞区室及其衍生物的特异性靶向。这项技术为研究体节发育的形态和遗传基础开辟了新的视角。发育动力学 229.643-650, 2004。(C) 2004 Wiley-Liss, Inc.
In ovo electroporation is a well-established method of gene transfer into neural and mesenchymal tissue in chick embryos. Electroporation of somites, however, has been hampered by low efficiency due to technical difficulties. Here, we present a powerful technique to electroporate avian somites and subpopulations; of somitic cells at high efficiency in ovo. We demonstrate specific targeting of distinct somitic compartments and their derivatives using single or combinations of plasmid expression vectors. This technique opens new perspectives to investigate the morphologic and genetic basis of somite development. Developmental Dynamics 229.643-650, 2004. (C) 2004 Wiley-Liss, Inc.