Seed-Mediated Synthesis of Polypeptide-Engineered Stabilized Fluorescence-Enhanced Core/Shell Ag2S Quantum Dots and Their Application in pH Sensing and Bacterial Imaging in Extreme Acidity
Seed-Mediated Synthesis of Polypeptide-Engineered Stabilized Fluorescence-Enhanced Core/Shell Ag2S Quantum Dots and Their Application in pH Sensing and Bacterial Imaging in Extreme Acidity
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种子介导的多肽工程稳定荧光增强核/壳 Ag2S 量子点的合成及其在极端酸性 pH 传感和细菌成像中的应用
DOI：
10.1021/acssuschemeng.9b02294
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发表时间：
2019
影响因子：
8.4
通讯作者：
Liu Bo
中科院分区：
文献类型：
--
作者：
Zhao Donghui;Yang Jie;Jin Ruimei;Hou Xiaolin;Li Chaoqing;Chen Wei;Zhao Yu;i;Wang Qiong;Liu Bo
pH值在生物系统中起着重要作用，荧光pH探针可以无创监测pH的变化和分布。在这里，我们介绍了一种基于基因工程多肽稳定的第二近红外荧光增强核/壳 Ag2S 量子点的新型 pH 探针。通过种子介导生长方法合成的核/壳Ag2S量子点在2.1-7.2范围内显示出荧光强度与pH值之间良好的线性关系。此外，核/壳Ag2S量子点表现出优异的可逆性和良好的生物相容性，可以作为革兰氏阴性菌成像和检测的多功能探针，在极酸性环境中不受自发荧光的影响。
The value of pH plays an important role in biological systems, and fluorescence pH probes enable noninvasive monitoring of the change and distribution of pH. Here, we introduce a new class of pH probe based on the genetically engineered polypeptide stabilized second near-infrared fluorescence-enhanced core/shell Ag2S quantum dots. The core/shell Ag2S quantum dots synthesized by seed-mediated growth methods showed a good linear relationship between fluorescence intensity and pH value in the range 2.1–7.2. In addition, the core/shell Ag2S quantum dots exhibited excellent reversibility and good biocompatibility, which can serve as multifunctional probes for the imaging and detecting of Gram-negative bacteria without the influence of autofluorescence in the extremely acidic environment.