Retinol status of newborn infants with congenital diaphragmatic hernia
Retinol status of newborn infants with congenital diaphragmatic hernia
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DOI：
10.1007/s003830050399
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发表时间：
1998-10-01
影响因子：
1.8
通讯作者：
Cloutier, R
中科院分区：
文献类型：
--
作者：
Major, D;Cadenas, M;Cloutier, R
先天性膈疝（CDH）的病因尚不清楚。 1941 年的文献研究报道，怀孕期间维生素 A 的营养缺乏可能会导致幼年大鼠患 CDH，与其他畸形相关或无关。最近，有人提出了细胞类视黄醇结合蛋白和视黄酸受体的表达模式与维生素 A 缺乏的形态学影响之间可能存在的相关性。本研究的目的是在人类新生儿中验证维生素 A 缺乏症与先前在实验动物中观察到的 CDH 之间可能存在的联系。在出生后的最初几个小时内，从 11 名足月 CDH 新生儿和 11 名与胎龄匹配的健康对照中采集血液样本，同时还从每组中的 7 名母亲处采集血样，总共 7 对匹配的 CDH 对照新生儿-母亲对。通过高效液相色谱法测量血浆视黄醇，通过比浊法测量视黄醇结合蛋白（RBP）。在 11 名匹配的 CDH 对照新生儿中，CDH 新生儿的血浆视黄醇和 REP 水平比对照值低 50%（分别为 P < 0.0002 和 < 0.006）；相反，CDH 母亲的视黄醇水平显着高于对照母亲（P < 0.005）。相对于对照组，CDH 婴儿的视黄醇和 REP 血浆浓度较低这一观察结果可能在临床上非常相关，并可能有助于阐明这种先天性异常的发生机制。
The etiology of congenital diaphragmatic hernia (CDH) is not yet known. Studies in the literature from 1941 have reported that nutritional deficiency of vitamin A during pregnancy could lead to CDH, associated or not with other malformations in young rats. More recently, possible correlations between expression patterns of cellular retinoid-binding protein and retinoic-acid receptors and morphologic effects of vitamin A deficiency have been suggested. The purpose of this study was to verify in human newborns the possible link between vitamin A deficiency and CDH previously observed in experimental animals. Blood samples were obtained during the first hours after birth from 11 term CDH newborns and 11 healthy controls matched for gestational age, and also from 7 mothers in each group, for a total of 7 newborn-mother pairs of matched CDH-controls. Plasma retinol was measured by high-performance liquid chromatography and retinol-binding protein (RBP) by nephelometry. In the 11 matched CDH-control newborns, plasma retinol and REP levels in CDH newborns were 50% less than control values (P < 0.0002 and < 0.006, respectively); in contrast, retinol levels in CDH mothers were significantly higher than those of control mothers (P < 0.005). The observation that the plasma concentrations of retinol and REP are low in infants with CDH relative to controls may be clinically very relevant and may help to elucidate the mechanism of development of this congenital anomaly.