Construction of a Nanosecond Fluorometric System for Applications to Biological Samples at Cell or Tissue Levels
Construction of a Nanosecond Fluorometric System for Applications to Biological Samples at Cell or Tissue Levels
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构建适用于细胞或组织水平生物样品的纳秒荧光系统
DOI：
10.1143/jjap.15.2433
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发表时间：
1976
影响因子：
1.5
通讯作者：
T. Kunii
中科院分区：
文献类型：
--
作者：
K. Kinosita;S. Mitaku;A. Ikegami;N. Ohbo;T. Kunii
描述了利用单光子计数技术的荧光系统，该系统记录脉冲激发后荧光强度的衰减和各向异性。对细胞悬浮液的宏观观察和在光学显微镜下对单个细胞的测量都是可能的。该系统的设计允许精确评估复杂的各向异性衰变，这对于细胞或组织的研究至关重要。数据通过正确分析这些复杂衰变的方法进行处理。对红细胞膜的初步研究证实了该系统的高分辨率和精确度。
A fluorometric system utilizing the single photon counting technique which records the decay of the intensity and the anisotropy of fluorescence following pulse excitation is described. Both macroscopic observations on cellular suspensions and measurements on individual cells under an optical microscope are possible. The system is so designed to permit precise evaluation of complex anisotropy decays, which is essential for studies of cells or tissues. Data are processed by a method which properly analyzes these complex decays. A preliminary study on erythrocyte membrane has confirmed the high resolution and precision of the system.