A novel electrochemiluminescent immunosensor based on the quenching effect of aminated graphene on nitrogen-doped carbon quantum dots.
A novel electrochemiluminescent immunosensor based on the quenching effect of aminated graphene on nitrogen-doped carbon quantum dots.
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DOI：
10.1016/j.aca.2015.07.018
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发表时间：
2015-08
影响因子：
6.2
通讯作者：
Jing Zhou;Tongqian Han;Hongmin Ma;Tao Yan;Xuehui Pang;Yueyun Li;Q. Wei
中科院分区：
文献类型：
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作者：
Jing Zhou;Tongqian Han;Hongmin Ma;Tao Yan;Xuehui Pang;Yueyun Li;Q. Wei
以二乙烯三胺五乙酸（DTPA）为原料，采用碳化法制备了平均粒径为2 nm的氮掺杂碳量子点（N-CQD）。制备简单的N-CQDs具有良好的电致化学发光（ECL）性能，并将其作为发光体制备了一种新型的ECL免疫传感器。还合成了胺化石墨烯（NH 2-G）并用作二抗的标记物。标记的NH 2-G能有效地猝灭修饰电极上N-CQDs的电化学发光，这是由于电化学发光共振能量转移（ERET）。诱导ECL淬灭的免疫识别使得能够定量测定生物标志物。甲胎蛋白（AFP）被选为模型分析物，研究所提出的免疫传感器的分析性能。在最佳条件下，甲胎蛋白浓度在0.01 ~ 100 ng mL− 1范围内与电化学发光强度呈良好的线性关系，检出限为3.3 pg mL−1。该电化学发光免疫传感器具有良好的稳定性、选择性和重现性。
Nitrogen-doped carbon quantum dots (N-CQDs) with an average diameter of 2 nm were synthesized by carbonization of diethylene triamine pentacetate acid (DTPA). The simple prepared N-CQDs showed excellent electrochemiluminescence (ECL) property and were used as luminophors to fabricate a sandwich-type ECL immunosensor. Aminated graphene (NH2-G) was also synthesized and used as a label of secondary antibody. The labeled NH2-G could effectively quench the ECL of N-CQDs modified on electrodes due to ECL resonance energy transfer (ERET). Immunological recognition which induced ECL quenching enabled the quantitative determination of biomarkers. Alpha fetoprotein (AFP) was selected as a model analyte to investigate the analytical performance of the proposed immunosensor. Under optimal conditions, a good linear relationship between ECL intensity and the logarithm of AFP concentration was obtained in the range of 0.01–100 ng mL−1with the detection limit of 3.3 pg mL−1. The proposed ECL immunosensor showed good stability, acceptable selectivity and reproducibility.